This document discusses the analytical method development and validation of prednisolone sodium phosphate using a quality by design (QBD) approach, aligning with ICH Q8(R2) guidelines. The study includes both spectroscopic and chromatographic methods, highlighting the use of UV spectrophotometry for determining the assay of the compound, with detailed methodology on solubility, stock solution preparation, precision, and stability testing. The research aims to establish a simple, rapid, and sensitive HPLC method for analysis, ensuring accuracy and reproducibility in measuring prednisolone sodium phosphate.