Learn LEP in 3 minutes | Leptin
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Leptin (lep) is a key cytokine involved in regulating energy balance and body weight, produced by the obese (ob) gene. It is primarily expressed in adipose tissues and functions as an appetite regulator in the hypothalamus. The document provides information on its structure, expected band sizes in Western blot analysis, and historical context related to its therapeutic potential for obesity management.
Learn LEP in 3 minutes | Leptin
- 1. www.bosterbio.com LEP IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best LEP antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Leptin (LEP) ○ Product of obese (ob) gene ● Long-chain helical cytokine ● Key regulator of energy balance and body weight ● An appetite regulator in the hypothalamus ● Mediates innate immunity ● Mature form: ○ 167 amino acids long ○ ~16-18 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P41159
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000174697-LEP/tissue
- 5. 03. LEP WESTERN BLOT 1,221 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=leptin+western+blot&report=imagesdocsum After reviewing several WB images, we observe LEP at ~16 kDa-18kDa. PMC7520685 Figure 4 PMC2970652 Figure 4 PMC3033396 Figure 2 Anti-Leptin Antibody Picoband™ (A00479- 3, rabbit polyclonal)
- 6. Douglas Coleman and Jeffrey Friedman discovered LEP. 04. HISTORY LEP has been a target of therapeutic interventions for obesity and weight loss management. 1994 CLINICAL POTENTIAL
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Editor's Notes
- #2: Does your next experiment involve Leptin? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against Leptin.
- #3: We will cover some basic facts, protein expression, and Leptin’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- #4: Topic 1: Quick facts about Leptin. Leptin is part of a family of long-chain helical cytokines. It binds to leptin receptors which are found throughout the body to mediate a variety of signaling pathways. The leptin protein is a product of the obese gene. It has been identified as a key regulator of energy balance and body weight, and acts as an appetite regulator in the hypothalamus. Furthermore, leptin mediates innate immunity by modulating the activity of neutrophils through increasing chemotaxis and secretion of oxygen free radicals. Leptin is conserved amongst mammals. It is 167 amino acids long and runs about 16 kilodaltons on Western Blot.
- #5: Topic 2: Where is Leptin expressed? High levels of leptin RNA have been found in adipose and breast tissues. They are also expressed at low levels in the placenta, skin, and appendix. You can find more information about tissue expression on protein atlas dot org as well as which cell lines express LEP. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- #6: Topic 3: Leptin in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically a single band can be expected for leptin in Western blot at approximately 16 to 18 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- #7: Topic 4: Some interesting facts about Leptin. Overall, Leptin has significant roles in the regulation of energy homeostasis, neuroendocrine function, and metabolism. Since its discovery in 1994 by Douglas Coleman and Jeffrey Friedman, leptin has raised excitement surrounding therapies for obesity and weight loss. In 1950, a recessive mutant colony of house mice kept in captivity was observed to have hyperphagia, decreased energy expenditure, and early onset obesity. It was later identified that this mice strain lacked the obese gene which encodes for leptin. In humans, circulating leptin levels modulate energy reserves and direct the central nervous system via the hypothalamus to adjust food intake and energy expenditure. Thus, leptin has been identified as an important protein for research on new drug candidates for obesity.