Learn CSF3/G-CSF in 3 minutes | Granulocyte colony-stimulating factor
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The document provides quick facts about granulocyte colony-stimulating factor (CSF3), a cytokine involved in hematopoiesis, with details on its structure, function, and tissues of expression. It also highlights the importance of CSF3 in treating neutropenia and includes references to Western blot data and antibody options. Key information includes a molecular weight of ~22.293 kDa and a protein length of 207 amino acids.
Learn CSF3/G-CSF in 3 minutes | Granulocyte colony-stimulating factor
- 1. www.bosterbio.com CSF3 IN 3 MINUTES Quick facts, control designs, Western Blot MW. Info you can use to find the best CSF3 antibody.
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. ● Granulocyte colony-stimulating factor (G-CSF or CSF3) ● A cytokine that acts in hematopoiesis ● Length of CSF3 protein is 207 amino acids ● Molecular Weight: ~22.293 kDa 01. FACTS AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P09919
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000108342-CSF3/tissue
- 5. 309 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc?term=g-csf%20western%20blot&report=imagesdocsum After reviewing several WB images, we observe CSF3 at ~23 kDa. 03. CSF3 WESTERN BLOT PMC5758815 Figure 2 Boster Anti-CSF3 Antibody (M02280-1, Rabbit Monoclonal) PMC8086136 Figure 2 PMC7196588 Figure 9 Boster Anti-CSF3 Antibody (PB9563, Rabbit Polyclonal)
- 6. CSF3 is part of the IL-6 superfamily of cytokines that regulates production, differentiation, and function of granulocytes and monocytes. 04. HISTORY CSF3 is used to treat neutropenia, a disorder characterized by an extremely low number of neutrophils in blood. 1983 CLINICAL POTENTIAL
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Editor's Notes
- #2: Does your next experiment involve CSF3? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against CSF3.
- #3: We will cover some basic facts, protein expression, and CSF3 expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- #4: Topic 1: Quick facts about CSF3. Granulocyte colony-stimulating factor, or CSF3, is a cytokine that acts in hematopoiesis. It controls the production, differentiation, and function of two types of white blood cells, granulocytes and monocytes or macrophages. The CSF3 protein is 207 amino acids long and runs at about 22 kilodaltons on Western blot.
- #5: Topic 2: Where is CSF3 expressed? High levels of CSF3 RNA have been found in the respiratory system and the bladder, with the highest levels detected in the lungs. It is also expressed at lower levels in the male and female tissues, muscles, connective tissues, and bone marrow. The protein is secreted and released outside the cell membrane. You can find more information on protein atlas dot org to check out which cell lines express CSF3 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- #6: Topic 3: CSF3 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see a single band in a CSF3 Western blot at around 23 kilodaltons. Check out the PMC link for more images, which is also provided in the description box.
- #7: Topic 4: Some interesting facts about CSF3. G-CSF or CSF3 is a member of the IL6 superfamily of cytokines that regulates production, differentiation, and function of granulocytes and monocytes. It was first recognized and purified in 1983 and was cloned in 1986. Scientists have explored the potential of CSF3 in treating neutropenia, a disorder characterized by an extremely low number of neutrophils in blood.