Mmb1 lec2 qb2013
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This document outlines the activities and requirements for a course on biotechnology. It includes lectures, presentations, exams, lab activities and field trips. There will be three exams covering introduction to techniques, applications of biotechnology, and international laws and guidelines. Students will work in groups to present on developing a GMO and create an exhibit for BioWeek. The course will also include virtual laboratory activities covering DNA extraction, PCR, gel electrophoresis, and microarrays. The last meeting will discuss isolating genes from plants and animals as well as human cloning and stem cell research.
Mmb1 lec2 qb2013
- 1. ACTIVITIES DATE ACTIVITY JULY 5 LAST PPT LECTURE JULY 9 VIRUTAL LABORATORY ACTIVITY JULY 12 FIRST EXAMINATION JULY 16 FREE DAY: LIBRARY WORK ON HOW TO DEVELOP YOUR GMO JULY 19-23 GROUP PRESENTATION (PPT) -What to Develop -Why Develop -How will you Develop -Dangers or Issues to Expect JULY 26 APPLICATION OF BIOTECHNOLOGY LECTURE SERIES
- 2. REQUIREMENTS • GMO DEVELOPMENT PRESENTATION • BIOWEEK EXHIBIT • FIELD TRIP PORTFOLIO • Expectations Before the Trip • Learnings During the Trip • Advocacy: Spreading knowledge of Biotechnology to Others
- 3. exams • Examination 1: INTRODUCTION TO TECHNIQUES IN BIOTECHNOLOGY • Examination 2: APPLICATIONS OF BIOTECHNOLOGY • Examination 3: LAWS AND INTERNATIONAL GUIDELINES CONCERNING BIOTECHNOLOGY • Take-home (online)
- 4. LAST MEETING: HOW WERE YOUR GENES ISOLATED? • Golden Rice • BT Corn • Edible Vaccine in Potato • GMO Soya • Recombinant Hepatitis B vaccine www.designingdigitally.com
- 5. GENE ISOLATION www.koihealth.org Source of Genes Primers will amplify target Gene of Interest Amplicon for “CONSTRUCT” www.invitrogen.com www.sciencedirect.com
- 9. TRANSFORMATION & SELECTION MARKERS bio.davidson.edu passel.unl.edu
- 11. CONFIRMATION OF EXPRESSION • HOW WILL YOU KNOW IF THE GENE THAT YOU INSERTED IS EXPRESSED? viswagenbiotech.com en.wikipedia.org igece.org
- 13. HOW ABOUT PLANTS? A plant part Is cultured Callus grows Shoots develop Shoots are rooted; plant grows to maturity clonal-solutions.com.au
- 14. DELIVERING GENES (Agrobacterium & The Gene Gun) apps.rhs.org.uk users.comcen.com.au
- 16. Cloning overview • Organismal cloning produces one or more organisms genetically identical to the parent that donated the single cell • may lead to production of stem cells for research and other applications • CLONE: genetically identical individuals produced from one parent toonrefugee.com
- 17. Cloning plants bbc.co.uk • A totipotent cell is one that can generate a complete new organism • Plant cloning is used extensively in agriculture
- 18. Cloning animals • Nuclear Transfer (e.g. BRANDY (2004) the cow and DOLLY (1997) the sheep) • the nucleus of an unfertilized egg cell or zygote is replaced with the nucleus of a differentiated cell • Embryo Splitting (production of high value herds and livestock breeds)
- 21. HUMAN CLONING & STEM CELLS Should we or Should we NOT?
- 22. 2 kinds of cloning ipscell.com
- 23. The ivf way to have a baby
- 26. READING ASSIGNMENT: Success stories and fail stories using stem cell therapy Be sure to Identify the points of success and points of failure thinkprogress.org
- 27. NEXT MEETING: the virtual laboratory PER GROUP: 1. Laptop 2. Internet Source
- 28. ACTIVITY THE VIRTUAL LABORATORY Main reference: http://learn.genetics.utah.edu
- 29. WE WILL NEED AN I-NET CONNECTION • http://learn.genetics.utah.edu/content/labs/ extraction/ • http://learn.genetics.utah.edu/content/labs/pcr/ • http://learn.genetics.utah.edu/content/labs/gel/ • http://learn.genetics.utah.edu/content/labs/ microarray/ • http://learn.genetics.utah.edu/content/tech/cloning/ clickandclone/
- 30. FOR DISCUSSION • What is the significance of DNA isolation? • When is a PCR useful? • Why do bands appear in an electrophoresis? • How can a microarray data reveal gene expression? • When do you think cloning is not indicated?
- 31. NEXT MEETING: EXAM exam number 1