![• Stage 3 : Tumor targeting and localization of
bacteriobot
• It is the chemotactic response of bacteriobot to the
[con] gradients of various of cancer cells :
• NIH/3T3 , CT_26 ( colorectal cancer )
• 4T1 ( breast cancer )
• Targeting measured by migration of bacteria out of
central region toward the side chambers
9/24/2015 22](https://image.slidesharecdn.com/nanorobotsedited-150924100835-lva1-app6892/85/Nano-robots-and-case-study-22-320.jpg)
![References
• [1]Sung Jun Park1, Seung-Hwan Park2, and et al. “New paradigm for tumor t
heranostic methodology using bacteria-based microrobot” Department of Nuclear
Medicine, Chonnam National University Medical School, Published,2 December
2013
• [2] Sylvain Martel, NanoRobotics Laboratory, Department of Computer and
Software Engineering, Institute of Biomedical Engineering, “NANOROBOTS FOR
MICROFACTORIES TO OPERATIONS IN THE HUMAN BODY AND ROBOTS PROPELLED
BY BACTERIA”
• [3] http://www.dailymail.co.uk/sciencetech/article-3000904/
• [4] Leslie Rubinstein, resident, Renaissance Technologies 2568 Woodhill Drive,
Lexington, KY, 40509 A Practical NanoRobot for Treatment of Various Medical
Problems
• [5]S. Brito Raj et al. (2012) Int J of Ad Biomed & Pharm Res., Depatment of
pharmaceutics, “NANOROBOTICS AND
THEIR PHARMACEUTICAL APPLICATIONS” 1(1): 43-54.
349/24/2015](https://image.slidesharecdn.com/nanorobotsedited-150924100835-lva1-app6892/85/Nano-robots-and-case-study-34-320.jpg)
Nano robots and case study
- 1. NanoRobots
- 2. Presented By- Leena Vaidya (112A5026) Guide:Mrs.Shubhangi Kadu 9/24/2015 2
- 3. Introduction Nanorobotics is the emerging technology field creating machines or robots whose components are at or close to the scale of a nanometer(10−9 meters). 9/24/2015 3
- 4. Types of nano robots • Nubot • Bacteria based • Virus based • Surface bound Systems 9/24/2015 4
- 5. Nubots • Also known as “DNA machine”. • A DNA machine is a molecular machine constructed from DNA. • Similar double helix structure like the DNA strands. 9/24/2015 5
- 6. Bacteria based micro robot • Uses a flagellum for propulsion purposes. • Use similar mechanisms as the biological microorganisms 9/24/2015 6
- 7. Bacteria based microrobot (bacteriobots) The bacteriobot can be considered as a new type of active Drug Delivery System with the various useful properties of a microorganism for synergistic therapy against incurable diseases 9/24/2015 7
- 8. Components of bacteriobots • Bacteria 1. These bacteria enable the micro robot to move toward tumors, acting as a combination of micro- sensor, micro-actuator, and therapeutic agent E.g. For microrobot actuation, microorganisms with high motility, such as Escherichia coli (E. coli), Salmonella typhimurium (S.typhimurium), Serratia marcescens (S. marcescens), 9/24/2015 8
- 9. Micro bead : The microstructure acts as a therapeutic molecule containing high amounts of drugs which plays important role in the treatment of tumor. 9/24/2015 9
- 10. Case 1:Bacteria based micro robot used in cancer treatment 9/24/2015 10
- 11. Introduction • Cancer is the disease caused by an uncontrolled division of abnormal cells in a part of the body • Chemotherapy , is the conventional treatment but.. •Damage growing-healthy normal cells blood and hair cells •Not specific and No continuous drug delivery for tissues Negative side effects ( anemia , nausea ) 9/24/2015 11
- 12. Bacteriobot robots • This research offer an alternative way instead of chemotherapy • Bacteria based micro robot ( Bacteriobot ) Bacteriobot = genetically engineered Bacteria + micro structure bead ( drug ) 9/24/2015 12
- 13. Why Nano robots ? • Specific .. target cancer cells but not normal cells • Depends on biodegradable and biocompatible materials • optimal drug quantities delivered to the target cells 9/24/2015 13
- 14. How bacteriobot works? • To be effective , bacteriobot moves toward tumors acting as combination of • Micro sensor • Micro actuator • Therapeutic agent 9/24/2015 14
- 15. Designing • Tumor model : colorectal cancer , breast cancer 4T1 • Animal model : CT_26 mouse • Bacteria model : ppGpp S. typhimurium strain SHJ2037 9/24/2015 15
- 17. How to combine Salmonella to capsule ? • To deliver drug to tumor cells • Salmonella work as mechanical engine • Micro capsule work as drug container 9/24/2015 17
- 18. Engineering the bacteriobot • It is a fusion between RT* and BT* presents a novel anti tumor strategy • Stage 1 : Interaction between biotin – streptavidin • Biotin found in all living organisms • Attenuated salmonella genetically modified to express biotin on the outer membrane of the cell * RT : Robot technology * BT : Biotechnology 9/24/2015 18
- 19. • High affinity interaction between biotin – streptavidin • Biotin can conjugate to many proteins easily let that protein to interact to with streptavidin conjugated molecules 9/24/2015 19
- 20. • Stage 2 : Development of flow-free chemotactic micro fluidic chamber • to evaluate targeting , motility , velocity of bacteriobot • This device composed of 3 chambers : Normal cells - Bacteriobots - tumor cells • Uses fluorescent dyes to see Nano robots target cells 9/24/2015 20
- 22. • Stage 3 : Tumor targeting and localization of bacteriobot • It is the chemotactic response of bacteriobot to the [con] gradients of various of cancer cells : • NIH/3T3 , CT_26 ( colorectal cancer ) • 4T1 ( breast cancer ) • Targeting measured by migration of bacteria out of central region toward the side chambers 9/24/2015 22
- 24. 9/24/2015 24
- 25. Methods: • Bacterial strains • Cell culture • Preparation of cell lysates • Fabrication of gradient microfludic device • Fabrication of bacteriobots • Animal models 9/24/2015 25
- 26. Detection: • Optical bioluminescence imaging • NIR imaging 9/24/2015 26
- 27. Case study 2:Microscopic DNA devices injected into a leukemia patient in a bid to destroy abnormal cells 9/24/2015 27
- 28. Advantages • Precise drug delivery • Easy removal • Biodegradable and biocompatible materials • Durability 9/24/2015 28
- 29. Disadvantages • Technological limitations • Security threats • Manufacturing cost 9/24/2015 29
- 30. Applications 9/24/2015 30 • TREATING ARTERIOSCLEROSIS
- 31. 9/24/2015 31 •BREAKING UP BLOOD CLOTS Applications
- 33. 339/24/2015 • This technology is still in research and development phase, only few primitive designs have been tested. • All of these current developments in technology directs humans a step closer to nanorobots and simple, operating nanorobots is the near future. Nanorobots can theoretically destroy all common diseases of the 2lst century thereby ending much of the pain and suffering. • Simple everyday illnesses can be cured without ever visiting the physician. Invasive surgery will be replaced by an operation carried out by nano-surgical robots. Although research into nanorobots is in its preliminary stages, the promise of such technology is endless.
- 34. References • [1]Sung Jun Park1, Seung-Hwan Park2, and et al. “New paradigm for tumor t heranostic methodology using bacteria-based microrobot” Department of Nuclear Medicine, Chonnam National University Medical School, Published,2 December 2013 • [2] Sylvain Martel, NanoRobotics Laboratory, Department of Computer and Software Engineering, Institute of Biomedical Engineering, “NANOROBOTS FOR MICROFACTORIES TO OPERATIONS IN THE HUMAN BODY AND ROBOTS PROPELLED BY BACTERIA” • [3] http://www.dailymail.co.uk/sciencetech/article-3000904/ • [4] Leslie Rubinstein, resident, Renaissance Technologies 2568 Woodhill Drive, Lexington, KY, 40509 A Practical NanoRobot for Treatment of Various Medical Problems • [5]S. Brito Raj et al. (2012) Int J of Ad Biomed & Pharm Res., Depatment of pharmaceutics, “NANOROBOTICS AND THEIR PHARMACEUTICAL APPLICATIONS” 1(1): 43-54. 349/24/2015