P glo presentation
- 1. http://www.spillerlab.com/mission/ http://www.rci.rutgers.edu/~crebb/CREBB%20Pictures/CREBB%20Web%20Photo%20A http://blogs.scientificamerican.com/guest-blog/2011/03/23/dressing-the-meat-of- lbum/pages/GFP-EColi_jpg.htm tomorrow/
- 2. • GFP is isolated from the jelly fish, Aequorea victoria • It has been modified to enhance is fluorescing characteristic • You will be working with GFP tomorrow!
- 3. GFP tagged Mice Mouse Embryo http://www4.utsouthwestern.edu/olsonlab/images.html http://www.bannination.com/comments/5047156/c1455973 Neuron Fly Kidney Cells http://gfp-cdna.seebyseeing.net/ http://sciencewise.anu.edu.au/articles/tthy http://captain-nitrogen.tumblr.com/post/9257522157/this-wormy-world-indeed
- 4. • Take the DNA from the jelly fish and add it to the DNA of the fish • These organisms are called transgenic organisms, because they have genes from another organism put into their DNA • Trans = across • Genic = genes
- 5. • We are going to create a transgenic bacteria that glows in the dark. • We will take the GFP gene from jellyfish and put it into E.coli bacteria E. coli
- 6. • Transformation is when a cell takes in and expresses a new piece of genetic material (DNA) E.coli bacterial cell • E.coli is a prokaryote bacteria • It replicates fast • Scientists use it as a “cloning factory” Bacteria DNA New DNA piece enters E.coli cell
- 7. • The new DNA we will be working with is called a plasmid • A plasmid is a circular piece of DNA, carrying one or more genes. • The plasmid we are working with is called pGLO New DNA piece enters E.coli cell
- 8. • Each of the colored regions on this plasmid, is a gene (DNA) • Each gene has its own function: • araC –“turns on” GFP, in presence of arabinose • GFP – green fluorescent protein (makes the organism glow) • bla – makes a protein that breaks down ampicillin • ampicillin – able to kill bacteria
- 9. Green Fluorescent Protein Beta protein breaks down ampicillin (can grow with ampicillin around) Bacteria DNA pGLO plasmid
- 10. • We will be growing E.coli onto LB agar plates • LB agar givse nutrients (food) for the bacteria • The LB agar plates will have: • Arabinose presence activates GFP • Ampicillin – which will kill any bacteria that does not have the pGLO plasmid • Remember, bla gene breaks down ampicillin
Editor's Notes
- #3: When I say GFP what does that mean?Which one is the order name? and which is the species name?How do you know?
- #5: Write down transgenic organisms…
- #6: What does transgenic mean again?
- #7: Bacteria can often take in floating pieces of DNA, and once inside the bacteria, the cell can often start to express the new traits from the new piece of DNA.
- #10: The e.coli bacteria now has all the traits of the plasmid