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Pythium: Introduction, Structure and
Reproduction
Presented by
Dr. E.Gayathiri
Department of Plant Biology and Plant Biotechnology
Guru Nanak College
LEARNING OBJECTIVES
• HABITAT
• SYMPTOMS OF PYTHIUM
• STRUCTURE OF PYTHIUM
• REPRODUCTION
Systematic position
• Phylum – Heterokonta
• Class - Peronosporomycetes
• Order - Pythiales
• Family - Pythiaceae
• Genus- Pythium
HABITAT
• 92 species (28 species from India)
• Cosmopolitan in distribution.
• Aquatic, terrestrial or amphibious.
• They are facultative parasite, saprophytic and
parasitic
Pythium are divided in two types
On basis of diseases that affect plant parts
In contact above the ground
parts
In contact with the soil
Common disease caused by Pythium
Damping off
Soft rot
Wheat rot or foot rot
Disease
stages
Post-
Emergence
damping-off
Pre-Emergence
damping-off
Source: Pythium Stock Photos & Pythium Stock Images – Alamy alamy.com
Symptoms of Pythium
seedling
• Seedlings become pale green
• Show a weak , shriveled place of girdle of
brown color on the stem near the surface of
the soil.
Root
• lack of small feeder roots
• brown lesions in the roots
• loss of cortex
Stem
• Cortical cells are killed (tissue weak)
• The infected tissues appear soft and water
soaked
Soybean seed and seedling diseases ...
extension.umn.edu Damping-off: Identifying and Controlling Pathogens in Transplant ...
Mycelium appears as White,
fluffy mass
Mycelium branched, inter- or
intracellular, multinucleate
Filament, Coenocytic
Haustoria are not produced
Structure of
Pythium
Cell wall is composed
of glucan and
cellulose
Cell wall encloses
vacuolated cytoplasm
with cell organelles
Glycogen is the
reserve food mater.
Structure of
Pythium
CELL WALL
MITOCHON
DRIA
ENDOPLAS
MIC
RETICULUM
NUCLEI
RIBOSOMES
OIL
GLOBULES
GLYCOGEN
Reproduction
Zoospore
Formation
Conidia
Formation
Aplanospore
Formation
Asexual Reproduction
Pythium
• Oogonia and antheridia
are produced
• They are very close to
each other.
• They are often present
on the same hypha.
• Antheridium is present
just below the oogonium
Sexual
Reproduction
-
Oogamous
type
Fertilization tube is formed, the male nucleus
from antheridium passes through this
fertilization tube and enters the oosphere.
Two nuclei fuse to form zygotic nucleus.
Oogonium is globose.
It contains a single oosphere.
surrounded by periplasm.
Young oosphere is multinucleate.
But later on it becomes uninucleate.
Antheridium is club shaped
and multinucleate in the
beginning.
But later on it becomes
uninucleate.
Fertilized egg secretes a thick wall
and changes into an oospore
Pythium
High temperature favours mycelium formation.
But low temperature favours the production of zoospores.
Mycelium are produced directly, or it may produce a short hypha.
This hypha develops a sporangium at its tip.
Reduction division takes place bcfore the germination of oospore
Oospore germinates after a period of rest
Germination
PYTHIUM GERMINATION
LIFE CYCLE OF PYTHIUM
Control of the Disease
Caused by Pythium
The soil treatment of
nursery beds by the use of
chemicals such as
formaldehyde diluted in
proportion of one part to
50 parts of water and
Bordeaux mixture ( ½
gallon per square foot)
To control pre-
emergence phase
of ‘damping off
Blitox-50 and
Arasan.
Thin sowing to
avoid over-
crowding
Adequate
exposure to air
and light of the
seed beds
Prevention of
excessively moist
conditions
Drenching soil
with 0.2%
Fytolan, 0.5%
Perenox and
0.2% Flit 406
Lowering the
humidity of the
atmosphere
Pythium

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Pythium

  • 1. Pythium: Introduction, Structure and Reproduction Presented by Dr. E.Gayathiri Department of Plant Biology and Plant Biotechnology Guru Nanak College
  • 2. LEARNING OBJECTIVES • HABITAT • SYMPTOMS OF PYTHIUM • STRUCTURE OF PYTHIUM • REPRODUCTION
  • 3. Systematic position • Phylum – Heterokonta • Class - Peronosporomycetes • Order - Pythiales • Family - Pythiaceae • Genus- Pythium
  • 4. HABITAT • 92 species (28 species from India) • Cosmopolitan in distribution. • Aquatic, terrestrial or amphibious. • They are facultative parasite, saprophytic and parasitic
  • 5. Pythium are divided in two types On basis of diseases that affect plant parts In contact above the ground parts In contact with the soil Common disease caused by Pythium Damping off Soft rot Wheat rot or foot rot
  • 7. Symptoms of Pythium seedling • Seedlings become pale green • Show a weak , shriveled place of girdle of brown color on the stem near the surface of the soil. Root • lack of small feeder roots • brown lesions in the roots • loss of cortex Stem • Cortical cells are killed (tissue weak) • The infected tissues appear soft and water soaked Soybean seed and seedling diseases ... extension.umn.edu Damping-off: Identifying and Controlling Pathogens in Transplant ...
  • 8. Mycelium appears as White, fluffy mass Mycelium branched, inter- or intracellular, multinucleate Filament, Coenocytic Haustoria are not produced Structure of Pythium
  • 9. Cell wall is composed of glucan and cellulose Cell wall encloses vacuolated cytoplasm with cell organelles Glycogen is the reserve food mater. Structure of Pythium
  • 13. • Oogonia and antheridia are produced • They are very close to each other. • They are often present on the same hypha. • Antheridium is present just below the oogonium Sexual Reproduction - Oogamous type
  • 14. Fertilization tube is formed, the male nucleus from antheridium passes through this fertilization tube and enters the oosphere. Two nuclei fuse to form zygotic nucleus. Oogonium is globose. It contains a single oosphere. surrounded by periplasm. Young oosphere is multinucleate. But later on it becomes uninucleate. Antheridium is club shaped and multinucleate in the beginning. But later on it becomes uninucleate. Fertilized egg secretes a thick wall and changes into an oospore
  • 16. High temperature favours mycelium formation. But low temperature favours the production of zoospores. Mycelium are produced directly, or it may produce a short hypha. This hypha develops a sporangium at its tip. Reduction division takes place bcfore the germination of oospore Oospore germinates after a period of rest Germination
  • 18. LIFE CYCLE OF PYTHIUM
  • 19. Control of the Disease Caused by Pythium The soil treatment of nursery beds by the use of chemicals such as formaldehyde diluted in proportion of one part to 50 parts of water and Bordeaux mixture ( ½ gallon per square foot) To control pre- emergence phase of ‘damping off Blitox-50 and Arasan. Thin sowing to avoid over- crowding Adequate exposure to air and light of the seed beds Prevention of excessively moist conditions Drenching soil with 0.2% Fytolan, 0.5% Perenox and 0.2% Flit 406 Lowering the humidity of the atmosphere