Running head PROJECT CHARTER ABOUT CREATING A WEBSITE FOR HR .docx

Running head: PROJECT CHARTER ABOUT CREATING A
WEBSITE FOR HR 1
PROJECT CHARTER ABOUT CREATING A WEBSITE FOR
HR 2
Project Charter about Creating a Website for HR
Student’s Name
University Affiliation
Project Charter about Creating a Website for HR
Introduction
To many of the business enterprises of today, establishment of a
project charter is of great importance towards the overall
prosperity of the given project. Through its creation, an
organization is at a position assessing the task and the
prospective outcomes of the given project. Similarly, it is a
component that ensures that the organization justifies its
mission statement in relation to the project. An organization is
also at a position of underlining scope statement of the project
as well as the regulation of the utmost deliverables. Last but not
least, a project charter ensures that the organization effectively
applies the technical requirements, assumptions as well as to the
constraints of the given project (Doraiswamy et al., 2012). In
this paper, it takes turn emphasizing more regarding a
description of a narrative project in conformance to all the
components essential for a viable project charter development.
Narrative Project Description
In this paper, it mainly focuses on a project charter for
Company Z that mainly operates in the production of cement.
Due to the increase in the overall turnout
of employees, the company resorts to up scaling its operations

through the establishment of a website for HR in conformance
to the assessment of employee’s records. Through the given
website, it enables the executives to effectively undertake
valuation of the employee’s records in relation to their
performance track at the company. Currently, the business is
experiencing a good customer base majorly because it deals in
one of the best cement variety in the construction industry. The
business has employed an array of employees hence the need to
go online in tracking employee’s performance is of great
importance
to the business.
Through the online portal management of employees will be
much easier for both the executive as well as to the junior
employees. In the spirit of exploration on the project plans of
opening up an online employee portal, this paper contributes a
lot regarding an effective project plan layout
.
Problem and Result Statement
In the given project, it has a requirement of directing the entire
employee’s performance in the online portal
. It includes daily update of each and every operation
undertaken by the workers. In the project, there are little
chances of any forthcoming defects based on the outcomes of
the project
(Goldstein et al., 2012). Each and every worker should be
conversant with the information being fed into the online portal
as it is a great tool to be used by the company in undertaking
benchmarking efforts
. In the project, there occur various forms of delivery failures
that are unforeseen. The project thereby ensures that all
variables are followed effectively to avoid any losses as well as
to any penalty that awaits failure of the project’s evaluation.
Mission Justification
Mission justification of the given project is persistent with both

the vision and mission
statements of the business. Validation of the mission of the
project asserts the business’s mandate to commit to serving the
employees in a just standard which displays the verdicts of the
employees
. In the given project, it prospects to motivate instants of
employee’s optimism as well as their happiness towards their
day to day operations. In the long run, the given project should
establish significance hence delivering a transition in running of
the business.
Project scope statement
The scope of this project is generally focused on the main
objectives
, the staff members involved, the time span that it will take to
implement the project as well as to the main aims of the project.
The main aim of this project involves satisfying the employee’s
needs in relation to the day to interventions that they undertake
in the organization (Marini et al., 2012). In the online portal, it
will feature expected activities from each and every employee,
arrival time at work premise, payment of the employee as well
as to the past work performance of the given employees.
Major Deliverables
Project Business Case
: According to the plan of establishing an online portal for the
employees, this kind of deliverable is essential as it entails all
the processes available for each and every proposal of the
projects.
Statement of Work
: It is a type of deliverable whose main purpose is to enable
precise definition of objectives and goals of the project hence
leading to the enhancement of safeguarding stakeholder
recognition (Pick, 2015).

Government Strategies
: In this deliverable, it basically outlines distinctive plans of the
government, in affiliation to the day to day running of the given
business.
Project Schedules
: This type of deliverable has got a main purpose of delineating
set timeline of the project. Further, it is an effective deliverable
as it enables designation of set timelines of each and every
phase of the project. In doing so, it enables running of the
project to align with the overall designated deadline of the
given project.
High-level Milestones for Significant Events
It mainly consists of the indicators that track the progression of
the project. In this project, it involves the creation of a website
for keeping track each and every employee’s performance. It
thereby means that the available indicators will probably vary in
conformance to each and every phase of the project. When it
comes to proposals as well as endorsements, the project should
deliver foundation before reaching any decision. There should
be the provision of acceptance as well as the limelight for each
and every task being executed by the project. For instance,
there should be a project kick off gathering to remind all the
stakeholders about the expectations of the project.
Applicable technical requirements, assumptions, or constraints
For the purposes of concluding the project successfully, it is of
great essence to assess both assumptions as well as to the
constraints of the project effectively. Examples of applicable
technical requirements include computer literacy as well as the
capability to effectively analyze each and every task criteria of
the employees (Pick, 2015). From the given project, an instance
of an assumption is the completion of the project within a

period of relatively two months. This time limit is assumed to
be enough for not only establishing of the website but also
feeding of the past employee performance data into the website.
In the project, there also occur constraints that might interfere
with the progress of the project. It includes restraint of cost,
roster of the employees, and availability of resources. The
above constraints should be well addressed at the beginning of
each and every phase of the project.
References
Doraiswamy, P., & Shiv, P. (2012). 50 top IT project
management challenges. Ely, Cambridgeshire: IT Governance
Pub.
Goldstein, P., Evans, H., & Facing History and Ourselves
National Foundation. (2012). A convenient hatred: The history
of antisemitism. Brookline, MA: Facing History and Ourselves.
Marini, A., & Talbi, M. (2014). Desertification and Risk
Analysis Using High and Medium Resolution Satellite Data:
Training Workshop on Mapping Desertification. Dordrecht:
Springer Netherlands.
Pick, J. B. (2015). Geographic information systems in business.
Norwood Mass: Books24x7.com.
Content
Met
Partially Met
Not Met
Comments:
A project charter is developed.
X

Good job.
Narrative project description is included.
X
I don’t have a good understanding of what this project is. I can
tell it is something related to an online portal for HR needs, but
not much beyond that.
Problem and result statement included.
X
No problem or result statements are present. What is the
problem that this project will alleviate? Be explicit. Keep your
paper focused on the problem and how your project will
alleviate that problem.
Mission justification is included.
x
No mission justification is present. There is a statement that
this aligns with the company’s mission and vision, but those are
never told. Regardless, this project need to relate to the true
business need of making money. What will happen if this
project is not launched? Ultimately, your project should either
boost revenue, reduce costs, or both. Explain how your project
will do that.
Project scope statement is provided.
X
Your scope section describes some of the things you want your
software to do, but it doesn’t describe what your project will
and will not do. Don’t forget to mention a few things that will

not be in the project.
Major deliverables are added.
X
One of these deliverables should be in the project charter. One
is something that the project and the company cannot provide.
This should list what the project itself will provide.
High-level milestones are included for significant events.
X
Milestones should be specific, not general. Don’t forget to use
dates or durations will milestones.
Applicable technical requirements, assumptions, or constraints
are provided.
X
Some of these are good, but there should be more to establish
boundaries to your scope, which is poorly defined.
The paper is 700- to 1,050 words in length.
X
You slightly exceed the word limit.
Total Available
Total Earned
5.5
3.0/5.5

Writing Guidelines
Met
Partially Met
Not Met
Comments:
The paper—including tables and graphs, headings, title page,
and reference page—is consistent with APA formatting
guidelines and meets course-level requirements.
X
Good job.
Intellectual property is recognized with in-text citations and a
reference page.
X
Good job.
Paragraph and sentence transitions are present, logical, and
maintain the flow throughout the paper.
X
Good job.
Sentences are complete, clear, and concise.
X
Good job.
Rules of grammar and usage are followed including spelling and
punctuation.
X

Good job.
Total Available
Total Earned
2.5
2.5/2.5
Assignment Total
#
8
5.5/8
Additional comments: There are some issues with this charter.
Writing a business paper is very different than writing an
English paper. Clearly explain the problem. We need to build a
new HR portal program because it has this impact on our
operations which has this impact on our finances of $X for the
year. This project will fix this problem by performing these
functions. These other related functions are beyond the scope
and will not be touched by the project team. It will cost $X
over $X years. Include all the required sections as stated in the
instructions. Feel free to use bullets and tables as appropriate.
�I don’t think this is the right word. Do you mean retention?
Or is it the turnout for new applicants?
�How so? Explain this. What is the connection to
profitability?

�After reading your narrative, I’m not entirely sure what your
project is.
�What is the problem in this? How does this tie to the
organizations true business need – making money?
�I don’t have this reference in front of me, but this must be
paraphrased incorrectly or taken out of context. There are
always opportunities for defects.
�Build on this What is the baseline comparison?
�You have not stated the vision or the mission. Regardless, it
needs to tie to profitability.
�What are these?
�What are these objectives?
�The business case should have been presented here.
�This is usually for contracting outsourced work.
�Is this a project being launched by the government? Earlier, it
was described as Company Z. You can’t make plans for the
government.

�You will need to make one of these for week 2.
�You should have specific milestones with dates or durations
here.
RESEARCH ARTICLE
Yap and Taz regulate retinal pigment epithelial cell fate
Joel B. Miesfeld1, Gaia Gestri2, Brian S. Clark1,*, Michael A.
Flinn1, Richard J. Poole2, Jason R. Bader1,
Joseph C. Besharse1, Stephen W. Wilson2 and Brian A. Link1,‡
ABSTRACT
The optic vesicle comprises a pool of bi-potential progenitor
cells from
which the retinal pigment epithelium (RPE) and neural retina
fates
segregate during ocular morphogenesis. Several transcription
factors
and signaling pathways have been shown to be important for
RPE
maintenance and differentiation, but an understanding of the
initial
fate specification and determination of this ocular cell type is
lacking.
We show that Yap/Taz-Tead activity is necessary and sufficient
for
optic vesicle progenitors to adopt RPE identity in zebrafish. A
Tead-

responsive transgene is expressed within the domain of the optic
cup
from which RPE arises, and Yap immunoreactivity localizes to
the
nuclei of prospective RPE cells. yap (yap1) mutants lack a
subset of
RPE cells and/or exhibit coloboma. Loss of RPE in yap mutants
is
exacerbated in combination with taz (wwtr1) mutant alleles such
that,
when Yap and Taz are both absent, optic vesicle progenitor
cells
completely lose their ability to form RPE. The mechanism of
Yap-
dependent RPE cell type determination is reliant on both
nuclear
localization of Yap and interaction with a Tead co-factor. In
contrast to
loss of Yap and Taz, overexpression of either protein within
optic
vesicle progenitors leads to ectopic pigmentation in a dosage-
dependent manner. Overall, this study identifies Yap and Taz as
key
early regulators of RPE genesis and provides a mechanistic
framework for understanding the congenital ocular defects of
Sveinsson’s chorioretinal atrophy and congenital retinal
coloboma.
KEY WORDS: Eye development, Ocular morphogenesis,
Zebrafish,
Tfec, Hippo signaling, Sveinsson’s chorioretinal atrophy,
Choroid
fissure, Coloboma, Directed differentiation of stem cells
INTRODUCTION
The neural retina (NR) and retinal pigment epithelium (RPE)

arise
from a common pool of progenitors during optic vesicle
development. Specifically, in fish, cells from the outer layer of
the
optic vesicle migrate around its margins, as cells within the
interior
invaginate to form the optic cup (Picker et al., 2009; Kwan et
al.,
2012; Heermann et al., 2015). Those cells that remain in the
outer
layer of the optic cup constitute RPE progenitors, whereas the
interior is made up of NR progenitors (Picker et al., 2009; Kwan
et al., 2012; Heermann et al., 2015). These two retinal
populations
are distinguishable long before overt functional differentiation,
as
the inner elongated cells of the NR are morphologically distinct
from the outer flattened prospective RPE cells.
Initially, optic vesicle progenitors express the same
transcription
factor-encoding genes (such as lhx2, pax6, six3, vsx2 and
rx1/2/3),
and signaling from surrounding tissues subsequently contributes
to
the regionalization of the optic vesicle into prospective RPE and
NR
domains (eye morphogenesis is reviewed in supplementary
material
Movie 1; Sinn and Wittbrodt, 2013; Fuhrmann et al., 2014).
Even
after RPE and NR domains are established, both cell
populations
maintain the ability to transdifferentiate (Sinn and Wittbrodt,
2013;

Fuhrmann et al., 2014). As eye development progresses,
multiple
signaling pathways (including BMP, FGF, Notch, WNT, SHH
and
TGFβ) continue to influence the expression of transcription
factors
that function in the differentiation and maintenance of the NR
and
RPE (Sinn and Wittbrodt, 2013; Fuhrmann et al., 2014).
Although
several transcriptional modulators (including Mitf, Otx and
β-catenin) have been implicated in RPE differentiation and
maintenance, none has yet been shown to mediate the initial
specification of RPE cell identity. Therefore, either
combinations of
known transcriptional regulators or novel factors must initiate
specification of the RPE. Here we provide evidence that targets
of
the Hippo signaling pathway are key regulators of RPE
specification.
The Hippo kinase signaling cascade widely regulates apoptosis,
proliferation and cell fate decisions during development by
controlling the localization and stability of the transcriptional
co-
activators Yes-associated protein 1 (Yap1, or more commonly
Yap)
and WW domain containing transcription regulator 1 (Wwtr1, or
more commonly Taz) (Varelas, 2014). Inactive Hippo signaling
results in nuclear localized Yap and Taz and an increase in
transcription of Yap/Taz target genes. The main nuclear binding
partners for Yap and Taz are the Tea domain (Tead)
transcription
factors. There are four Tead homologs in vertebrates, which
together
are broadly expressed across tissues during development (Mann

et al., 2007; Naye et al., 2007).
An involvement of Yap and Tead in eye development is
suggested by the prominent expression of a Yap/Taz-Tead-
responsive transgene in tissues and cells undergoing complex
morphogenetic movements, including the eyes (Miesfeld and
Link,
2014). Furthermore, heterozygous loss-of-function mutations in
YAP1 in humans can result in autosomal dominant coloboma
and a
mutation within the Yap-binding domain of TEAD1 causes
Sveinsson’s chorioretinal atrophy (SCRA), an autosomal
dominant loss of RPE, choroid, and photoreceptors radiating
from the optic nerve head (Fossdal et al., 2004; Williamson et
al.,
2014). Although these mutations and associated diseases have
been described, the mechanism(s) underlying the defects is
unknown.
In this study we address the roles of Hippo signaling
components
during zebrafish eye development. We analyzed loss-of-function
mutations in both yap and taz, transgenic lines that manipulate
Yap
and Taz activity in a tissue-specific manner, and reporter lines
thatReceived 22 October 2014; Accepted 10 July 2015
1Department of Cell Biology, Neurobiology and Anatomy,
Medical College of
Wisconsin, Milwaukee, WI 53226, USA. 2Department of Cell
and Developmental
Biology, UCL, London WC1E 6BT, UK.
*Present address: Johns Hopkins University, Solomon Snyder
Department of
Neuroscience, 733 N. Broadway, Baltimore, MD 21205, USA.

‡Author for correspondence ([email protected])
This is an Open Access article distributed under the terms of the
Creative Commons Attribution
License (http://creativecommons.org/licenses/by/3.0), which
permits unrestricted use,
distribution and reproduction in any medium provided that the
original work is properly attributed.
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© 2015. Published by The Company of Biologists Ltd |
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http://creativecommons.org/licenses/by/3.0

label RPE progenitors. These tools revealed roles for the Yap
and
Taz transcriptional co-activators in choroid fissure closure and
RPE
specification that are likely to be conserved between zebrafish
and
humans.
RESULTS
Yap/Taz-Tead signaling is active during optic cup
morphogenesis
Analysis of the 4xGTIIC:d2GFP transgenic line (Miesfeld and
Link,
2014) suggested a role for Yap/Taz-Tead activity in the
developing
lens, NR and RPE (Fig. 1; supplementary material Movie 2).
Reporter transgene expression was evident in the ectoderm
overlying
the optic vesicle at 14 h post-fertilization (hpf) (Fig. 1A′) and in
the
lens placode and presumptive RPE by 18 hpf (Fig. 1B′).
Throughout
optic cup invagination transgene activity was present at low
levels in
NR progenitors and more prominently in RPE progenitors (Fig.
1;
supplementary material Movie 2). Fluorescence is first evident
in the
optic cup midway through its morphogenesis, but Yap/Taz-Tead
activity is likely to initiate earlier than this given the delay
between
transcription of the transgene and fluorescence of its protein
product.
To investigate the roles of Yap and Taz, we generated mutations
within each gene and analyzed the consequences for eye

formation.
yap mutants lack RPE cells
yap mutant alleles were generated using transcription activator-
like
effector nuclease (TALEN) technology. Multiple founders
containing
Fig. 1. A Yap/Taz-Tead reporter transgene is dynamically
expressed during optic cup morphogenesis and yap−/− mutants
exhibit RPE defects.
(A-D′) Images of live zebrafish from 14-24 hpf showing optic
cup development and 4xGTIIC:d2GFP transgene expression
(green). Arrows indicate cells that are
expressing the transgene while undergoing morphogenesis. (E)
Schematic of wild-type and mutant Yap and Taz. Yap S54 is an
essential residue for Tead binding
and S87 is phosphorylated by Lats leading to cytoplasmic
retention. The Yap c.158_161del and Taz c.156_160del mutants
contain frameshifts resulting in early
stop codons. TEAD BD, Tead transcription factor binding
domain (light blue); WW, dual tryptophan motif (green); TAD,
transactivation domain (fushia); PDZ
domain (dark blue). (F,G) yap−/−; taz−/− embryos arrest by 18
hpf with multiple defects. (H-J″) Live embryos (H-J′) and
sections (H″,I″,J″) of yap−/−; taz+/+ (I-I″) and
yap−/−; taz+/− (J-J″) showing RPE defects and additional NR
defects in yap−/−; taz+/− mutants (J′) compared with control
(H-H″). Boxed areas indicate locations of
TEM analysis. (K-L′) Transmission electron microscopy
analysis showing areas of normal RPE development (L′) and
areas devoid of RPE (L) in yap−/− eyes.
Asterisk indicates the presence of primary cilia on
neuroepithelial cells. L, lens; OV, optic vesicle; NR, neural
retina; RPE, retinal pigment epithelium; SE, surface
ectoderm; POM, periocular mesenchyme; NP, neuropil; PhRP,

photoreceptor progenitors.
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RESEARCH ARTICLE Development (2015) 142, 3021-3032
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different insertion or deletion alleles were obtained and two
lines
established. A 4 nt deletion, yap c.158_161del
(yapmw48/mw48,
referred to as yap−/−), resulted in a frameshift within the Tead-
binding domain of Yap leading to a predicted incorrect amino
acid
sequence and an early stop codon (Fig. 1E). yap−/− embryos
had a 3-
fold decrease in yap mRNA (P=0.0009) and lacked Yap

immunoreactivity (Fig. 3A-C), suggesting nonsense-mediated
mRNA decay and the absence of Yap protein.
By 1 day post-fertilization (dpf), yap−/− mutants show mild
heart
edema, vascular hemorrhages and an impairment in RPE
development (Fig. 1I-I″,L; supplementary material Fig. S1; data
not shown). Some yap−/− fish survived to adulthood and none
of the
early phenotypes were exacerbated through the loss of maternal
Yap
contribution in embryos generated from yap−/− mothers.
Embryos
heterozygous for the mw48 or other mutant alleles described
here
appeared overtly normal.
The loss of RPE in yap−/− mutants is noticeable as soon as
melanization begins and becomes more apparent once retinal
pigmentation is complete (Fig. 1I′,I″; supplementary material
Fig. S1). RPE deficiency typically occurs at the back of the eye
but can also variably occur on the lateral and ventral surfaces
and
can differ in phenotypic extent between eyes of the same
embryo.
Electron microscopy of 2 dpf yap−/− eyes revealed normal RPE
cells
in regions with visible pigmentation (Fig. 1L′). However, in
areas
lacking pigmentation there was an absence of flattened cells
characteristic of either RPE or periocular mesenchyme, and NR
progenitors directly abutted the forebrain neuropil (Fig. 1L).
The
retinal neuroepithelia appeared normal, possessed the modified
primary cilia that form photoreceptor outer segments, and

displayed
proper retinal layering, even beneath regions lacking RPE (Fig.
1I″).
yap mutants exhibit variable phenotypes including
coloboma
Although fully penetrant, the RPE phenotype in yap−/− mutants
was
variable and other phenotypes, including viability, showed
similar
variability. Additional support for phenotypic variability in yap
mutants came from assessment of another allele, nl13, which
exhibited a colobomatous phenotype (Fig. 2G-H) and was
identified
through a forward genetic screen of ENU-induced mutations.
The
nl13 mutation was localized between Zv2560 and Zv8353 on
chromosome 18 using bulked segregant analysis with SSLPs.
yap
lies within this interval and, given that mutations in human
YAP1
Fig. 2. yapnl13/nl13 mutants exhibit coloboma. (A) Splice site
variants elicited by the yapnl13 allele. The mutation results in
aberrant splicing and an early stop
codon in the transactivation domain for all described products.
(B) Protein schematics for wild-type and predicted mutant
yapnl13 allele variants. (C) Western blot
showing the loss of full-length Yap in yapnl13/nl13 mutants. A
smaller protein product is detectable at higher levels in the
mutant (∼40 kDa). (D-D″,G-G″) 3 dpf wild-
type and yapnl13/nl13 embryos showing coloboma (arrows) in
the absence of other overt phenotypes. Plastic sections of wild-
type (D″) and mutant (G″) eyes show
the coloboma (arrow) phenotype and RPE deficits that are
sometimes observed in the ventral retina. (E,F,H,I) Sections

showing Yap and Taz proteins (green) in
wild-type and yapnl13/nl13 mutant eyes. Red counterstain (TO-
PRO3) shows nuclei.
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can lead to isolated and syndromic coloboma (Williamson et al.,
2014), this gene was a good candidate for harboring the
mutation.
The genomic mutation was identified as a single base change
from
A to T in the splice acceptor site of intron 4. Sequencing the

coding
region from mutant cDNA revealed four splice variants (Fig.
2A,B),
with the main isoform resulting in a deletion of 11 nt between
positions 673 and 684, generating a stop codon at amino acid
309, the beginning of the transactivation domain (Fig. 2A,B).
Yap immunoreactivity was still detected in yapnl13/nl13
mutants
(Fig. 2E,H) but western blots showed the presence of a smaller
than
wild-type protein (∼40 kDa versus ∼65 kDa; Fig. 2C).
To test whether the mutation in yap caused the coloboma
phenotypes, we injected synthetic yap-GFP RNA into embryos
from a cross between carriers and assessed phenotypic rescue.
In
non-injected controls, 25.45% (n=28/110) of embryos showed
coloboma, whereas injection of 200 pg wild-type yap mRNA
rescued the coloboma phenotype (n=106/110 normal; 4/110
showed coloboma). As in humans with YAP1 mutations, the
coloboma phenotype in yapnl13/nl13 zebrafish embryos could
be
either unilateral or bilaterally symmetric. RPE deficits were
observed in yapnl13/nl13 embryos as in other alleles, but these
were
restricted to the ventral eye around the open choroid fissure.
Also, as
in other alleles, NR lamination was generally normal although
outer
retinal neurons were significantly disrupted where the coloboma
was present (Fig. 2G-G″). Other than the ventral eye
phenotypes,
yapnl13/nl13 embryos showed no overt phenotypes (Fig. 2G).
taz mutant alleles enhance the yap−/− phenotype

The variable loss of RPE in yap−/− embryos (and in other
alleles)
can be rescued by raising embryos at 20.5°C (supplementary
material Table S1). Together with the observation that some
RPE
develops in mutants, this suggests that another factor(s)
contributes
Fig. 3. yap mRNA and Yap protein levels are decreased and Taz
protein increased in yap−/− embryos. (A-B‴) Yap
immunoreactivity in wild-type and yap−/−
eyes at 28 hpf. Yap protein is present in flattened RPE nuclei
(arrows) and periocular mesenchyme (POM) in yap+/− embryos,
whereas nuclear Yap staining is
absent in the yap−/− mutant. (C) qRT-PCR analysis of whole
embryos at 32 hpf showing a decrease in yap (3-fold,
*P=0.0002) and taz (1.5-fold, *P=0.0270)
mRNA in yap−/− mutants. Dotted line indicates normalized
expression levels of yap and taz in wild-type embryos. An
unpaired t-test was performed and statistical
significance determined using the Holm-Sidak method. Error
bars represent s.e.m. (D) Western blot showing Taz protein
(∼52 kDa) in wild-type and its absence in
taz−/− adult heart tissue. (E-G‴) Taz immunoreactivity in wild-
type, taz−/− and yap−/− embryos at 28 hpf. (H) Western blot of
Taz protein from 2 dpf wild-type (n=20)
and yap−/− mutant (n=20) whole embryos.
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to RPE development. An obvious candidate is Taz, a
homologous
transcriptional co-regulator, and so we generated a mutant
allele with a 5 nt deletion, taz c.156_160del (tazmw49, referred
to
as taz−/−). This mutation leads to a frameshift deletion in the
Tead-
binding domain of Taz and a resultant truncated protein
containing a
sequence of 82 incorrect amino acids and an early stop (Fig.
1E).
Despite lacking detectable Taz protein (Fig. 3D), taz−/−
embryos
have no overt embryonic phenotype and survive to adulthood.
Although taz−/− RPE appears normal, the inclusion of one
mutant
taz allele within a yap−/− background (yap−/−; taz+/−)
enhanced the

loss of RPE and led to more severe body axis, heart and NR
defects
compared with yap−/− siblings (Fig. 1J-J″). Double-
homozygous
mutant (yap−/−; taz−/−) embryos arrest before eye
morphogenesis is
complete, precluding assessment of RPE development (Fig.
1F,G).
Unlike in yap−/−; taz+/+ embryos, rearing yap−/−; taz+/−
embryos
at low temperatures did not rescue the RPE phenotype
(supplementary material Table S2), suggesting that the
temperature sensitivity of the yap−/− phenotype is due to
redundancy with Taz (as also described in other situations;
Nishioka et al., 2009). Analysis of taz mRNA levels within
yap−/−
embryos did not reveal compensatory changes in transcript
abundance (Fig. 3C). However, Taz immunoreactivity appeared
increased at 28 hpf (Fig. 3G-G‴), higher total Taz protein levels
were detected in 2 dpf yap−/− embryos (Fig. 3H) and nuclear
localization appeared enhanced in yapnl13/nl13 mutants (Fig.
2F,I).
Nuclear activity of Yap/Taz-Tead is required for RPE genesis
SCRA is an autosomal dominant congenital disorder
characterized
by loss of RPE and photoreceptors proximal to the optic nerve
head
(Fossdal et al., 2004; Jonasson et al., 2007) and is caused by a
tyrosine-to-histidine mutation in the Yap-binding domain of
TEAD1 (Kitagawa, 2007). yap−/− embryos share phenotypic
features of SCRA patients with TEAD1 deficiencies, suggesting
that the consequences of Yap deficiency are mediated through
Tead.

In order to determine if a lack of Yap-Tead activity is
responsible
for RPE loss, we first tested whether the Yap and Tead binding
domains are conserved in zebrafish. We generated plasmids
encoding wild-type Tead1a, a mutant version equivalent to the
YAP1 binding-deficient allele of SCRA patients (Tead1a
Y417H),
wild-type Yap, and a variant with a mutation within the putative
Tead-binding domain (Yap S54A) (Zhao et al., 2008; Chen et
al.,
2010) (Fig. 4A). Consistent with studies in other species,
transfection assays in HEK293 cells showed that zebrafish Yap
and Tead1a are able to interact with each other, whereas the
mutant
variants Yap S54A and Tead1a Y417H are unable to bind (Fig.
4B).
Additionally, overexpression of the autosomal dominant Tead1a
Y417H allele within the optic vesicle resulted in RPE loss
around the optic nerve, similar to observations in SCRA
patients
(Fig. 4C-E′).
To further investigate the role of Tead in Yap-mediated RPE
genesis, we generated a Tead domain-specific yap mutant allele.
Fig. 4. Yap and Tead1a zebrafish protein interactions are
conserved. (A) Schematic of the zebrafish Yap and Tead
binding domain (BD) interaction sites.
(B) Immunoprecipitation of zebrafish Yap and Tead1a wild-type
and Tead-binding-deficient Yap (Yap S54A) and Yap-binding-
deficient Tead1a (Tead1a Y417H)
isoforms. All the mutated protein variants lose the ability to
interact, in contrast to the wild-type proteins.
Immunoprecipitation (IP) was with an anti-Flag antibody.
(C-E″) Whole eyes (C-D′) and sections (E-E″) showing RPE

loss surrounding the optic nerve head after overexpression of
Tead1a Y417H. Photoreceptors are red
owing to late expression of the rx3:Gal4 driver in these cells.
Arrows indicate areas lacking RPE.
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A c.158_178del (yapmw69/mw69, referred to as yapΔTB/ΔTB)
deletion
in yap created a seven amino acid in-frame deletion that
eliminates
the critical S54 and surrounding amino acids (Chen et al., 2010)
within the Tead-binding domain (Fig. 5A). yapΔTB/ΔTB
mutants had
slightly lower levels of yap mRNA (Fig. 5E). Although Yap
protein

was still detected (Fig. 5F-G‴) in yapΔTB/ΔTB embryos, loss of
RPE
was still evident (Fig. 5B-D).
Additional evidence for a nuclear role of Yap in RPE genesis
came from analysis of the consequences of overexpression of
dominant-negative forms of Yap and Taz that contain nuclear
localization sequences but lack transactivation domains (NLS-
YapDN, NLS-TazDN) (Cao et al., 2008; Miesfeld and Link,
2014).
When either dominant-negative Yap or Taz was mosaically
overexpressed in RPE/retinal precursors using vsx2:Gal4
(Miesfeld and Link, 2014) or rx3:Gal4, loss of RPE was
observed
(Fig. 8E-G; supplementary material Fig. S2). By contrast,
expression of either dominant-negative protein in periocular
mesenchymal cells using a foxc1b:Gal4 driver did not result in
RPE defects (not shown). Collectively, these results suggest
that
Yap/Taz-Tead-dependent transcription is required autonomously
within optic cup progenitor cells to generate RPE.
Optic vesicle proliferation and apoptosis are unaffected in
yap−/− mutants
Two well-reported functions of the Yap/Taz-Tead interaction
are the
control of cell proliferation and death. However, therewas no
obvious
difference in proliferation or cell death in yap−/− eyes at 14
hpf,
before markers of pigmentation are detected, at 18 hpf, the
onset of
observed Yap/Taz-Tead reporter transgene activity, or at 24 hpf,
when RPE loss is first detected (Fig. 6A,B). To more
specifically
assess the proliferation of prospective RPE cells, we used a

−2.7 kb
tfec:eGFP transgene that is expressed (as is the tfec gene; Lister
et al.,
2011) in presumptive and definitive RPE cells (Fig. 6C-E‴;
supplementary material Movies 3 and 4). When analyzed at 14
hpf,
there was no difference in the numbers of proliferating −2.7 kb
tfec:
eGFP-positive cells between yap−/− and sibling controls (Fig.
6F).
To further test the potential significance of cell death and
proliferation as contributors to the mutant phenotype, each
process
was inhibited during optic cup morphogenesis. To ameliorate
cell
death, yap mutants and their wild-type siblings were injected
with
tp53 morpholino (Robu et al., 2007) and bcl-xl mRNA (Sidi et
al.,
2008). Although normal apoptosis in the lens epithelium was
inhibited in all embryos, the yap mutant RPE phenotype was not
Fig. 5. Tead-binding-deficient yapΔTB/ΔTB mutants lack RPE
but maintain yap mRNA and Yap protein levels. (A) The Tead-
binding-deficient yapΔTB/ΔTB
zebrafish mutant. (B-D) 48 hpf whole eyes showing that yap−/−
and yapΔTB/ΔTB mutants lose a subset of RPE cells. Dashed
lines indicate the border of the eye.
(E) qRT-PCR analysis of whole embryos at 32 hpf revealing a
decrease in yap (1.6-fold, *P=0.0052) and taz (1.15-fold,
*P=0.0038) mRNA in yapΔTB/ΔTB mutants.
Dotted line indicates the normalized expression levels of yap
and taz in wild-type embryos. An unpaired t-test was performed
and statistical significance was

determined using the Holm-Sidak method. Error bars indicate
s.e.m. (F-G‴) Yap protein expression in yap+/ΔTB (F-F‴) and
yapΔTB/ΔTB (G-G‴) at 28 hpf. Yap
protein is present in flattened RPE nuclei (arrows) and
periocular cells in yap+/ΔTB and yapΔTB/ΔTB embryos.
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rescued (not shown). Additionally, impairing proliferation in
wild-
type embryos from 10.5 to 26 hpf with hydroxyurea and
aphidicolin

did not phenocopy the RPE loss seen in yap mutants (not
shown).
These results suggest that neither changes to proliferation nor
cell
death are major contributing factors to RPE loss and, therefore,
that
Yap activity may impart RPE identity upon bi-potential optic
vesicle cells.
Yap and Taz are essential for the generation of RPE
Our results suggest that Yap/Taz function might be essential for
cells
to adopt RPE identity; however, our genetic analyses did not
allow us
to definitivelyshow this becauseyap−/−; taz−/− embryos arrest
before
RPE begins to pigment. Consequently, to test whether retinal
precursors lacking Yap and Taz could form RPE, we
transplanted
fluorescently labeled yap; taz mutant cells into albino embryos
that
carried wild-type yap and taz alleles. Albino embryos can
generate
xanthophores, iridophores and leucophores but not the melanin
pigment found in RPE cells (Streisinger et al., 1986;
Tsetskhladze
et al., 2012). Therefore, we could easily assess whether the
fluorescently labeled optic vesicle cells of various yap; taz
genotypes were able to generate RPE (Fig. 7A-C′). We scored
the
presence/absence of pigmented RPE in eyes that contained
clones of
transplanted cells in the NR to ensure that transplanted mutant
cells
were properly targeted to the eye field of the host

blastula/gastrula.
In mosaic analyses, cells of all genotypes contributed to both
NR
and RPE, except for yap−/−; taz−/−, which only contributed to
NR
Fig. 6. Cell death and proliferation
are normal in yap−/− eyes. (A) The
numbers of dying cells identified by
Acridine Orange staining do not differ
in yap−/− mutants at 14 (P=0.8465),
18 (P=0.6542) or 24 (P=0.2558) hpf
as compared with wild type. Numbers
of eyes analyzed: 14 hpf, wt n=31,
yap−/− n=5; 18 hpf, wt n=39, yap−/−
n=10; 24 hpf, wt n=24, yap−/− n=16.
(B) Eye field mitotic cell counts do not
differ between yap+/? (wt) and yap−/−
at 14 (P=0.9205), 18 (P=0.4329) and
24 (P=0.2222) hpf. Numbers of eyes
analyzed: 14 hpf, wt n=38, yap−/−
n=6; 18 hpf, wt n=38, yap−/− n=6;
24 hpf, wt n=36, yap−/− n=6.
(C-E‴) Time-course of expression of
tfec:eGFP in prospective RPE cells.
(F) Mitotic cell counts of tfec:eGFP+
cells do not differ between wild-type
and yap−/− eyes at 14 hpf
(P=0.5408). Numbers of eyes
analyzed: wt n=18, yap−/− n=6. P-
values were obtained using an

unpaired t-test with equal s.d. Error
bars indicate s.e.m. Wild type
included full RPE coverage, whereas
yap−/− showed some RPE loss.
Fig. 7. yap−/−; taz−/− transplanted cells do not contribute to the
RPE. (A) Methods used to analyze the contribution of
transplanted cells to the RPE and NR.
(B-C″) Examples of transplanted wild-type (B-B″) and yap−/−;
taz−/− (C-C″) cells in 48 hpf eyes. The arrow in B indicates a
pigmented transplanted cell forming
RPE in the albino host; other black/white arrows indicate H2a-
GFP+ clones in NR.
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(Table 1). yap−/−; taz−/− cells were able to form neural crest-
derived

melanocytes indicating that the melanization pathway is
functional
(data not shown). These results suggest Yap and Taz are
essential for
optic vesicle cells to contribute to the RPE.
Complementing the loss-of-function analyses, vsx2:Gal4-driven
overexpression of wild-type Yap, constitutively active Yap (Yap
S87A) and, to a lesser extent, wild-type Taz, in retinal
progenitors
led to ectopic pigmentation as well as disrupted lamination
within
the NR at 5 dpf (Fig. 8A-D′). To determine if the morphological
changes were reflected in altered retinal mRNA expression
profiles
we sequenced RNA from 36 hpf eyes overexpressing Yap S87A,
which generated the strongest ectopic RPE phenotype. The top
20
transcripts upregulated by Yap S87A included cyr61 and ctgf,
which are established targets of Yap-Tead signaling, and both
were
verified as upregulated by RT-qPCR (supplementary material
Table S3, Fig. S3). Other upregulated mRNA transcripts
included
many implicated in RPE and pigment cell genesis,
differentiation
and function (Table 2). Upregulation of the RPE-specific dct
gene
was verified by in situ hybridization in 48 hpf retinae
(supplementary material Fig. S4A-B″). Additionally,
destabilized
GFP expressed from a ctgf promoter transgene (−1.0 kb ctgfa:
d2GFP) showed increased fluorescence in RPE cells and ectopic
expression in the NR (supplementary material Fig. S3B-F″) in
response to vsx2-mediated Yap overexpression.

The vsx2:Gal4 transgene is mosaically expressed in RPE
progenitor cells as well as NR progenitors (Miesfeld and Link,
Fig. 8. Overexpression of gain- and loss-of-
function yap transgenes alters RPE and NR cell
fate. (A-D′) Images showing that overexpression of
wild-type Yap (B,B′), constitutively active Yap (Yap
S87A) (C,C′) and Taz (D,D′) induces ectopic
pigmentation and retinal disorganization at 5 dpf, as
compared with the control (A,A′). Arrows indicate the
presence of ectopic pigment cells. (E-G) Mosaic
overexpression of dominant-negative forms of Yap
(F) and Taz (G) results in ectopic loss of RPE cells at
48 hpf, as compared with the control (E). Arrows
indicate areas devoid of RPE cells. (H) Model of
zebrafish RPE development. Bipotent progenitor
cells assume either an RPE or NR fate based on
Yap/Taz activity. In the absence of yap (right
hemisphere of the eye cup), progenitors contributing
to the central retina cannot form RPE. Those
progenitors that contribute to the peripheral eye cup
can upregulate Taz during their more lengthy
migration and therefore form RPE.
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2014) and thus if exogenous Yap were to trigger the migration
of
existing RPE cells into the NR, this could contribute to the
phenotype. However, RPE-specific −2.7 kb tfec:Gal4-driven
overexpression of Yap S87A did not lead to any obvious
migration
of Yap S87A-positive RPE cells into the NR (supplementary
material
Movies 5 and 6, Fig. S4C-C‴). Altogether, these results support
the
idea that Yap and Taz are necessary and sufficient to promote
optic
cup cells to adopt RPE cell fate.
DISCUSSION
Our study suggests a model for RPE genesis in which strong
Yap-
Tead transcriptional activity within bipotential optic cup
progenitors

drives RPE fate. Strong expression of the Yap/Taz-Tead-
responsive
4xGTIIC:FP transgene in presumptive RPE suggested a
potential
role for the downstream transcriptional components of the
Hippo
signaling pathway in RPE development. Although the
4xGTIIC:FP
transgene reports both Yap and Taz activity, Yap appears to be
the
main regulator of RPE cell fate. Consistent with this idea,
yap−/−
embryos are devoid of a subset of RPE cells, whereas taz−/−
embryos develop normal RPE. The difference in phenotypes is
consistent with the low levels of Taz expression in optic vesicle
cells. Although taz−/− embryos develop normal RPE cells,
compromising Taz function on a yap−/− background enhanced
the
loss of RPE cell phenotype as compared with yap−/− mutants
alone.
These observations place Yap and Taz as key regulators of RPE
fate
determination.
An interesting facet of the RPE phenotype in yap−/− embryos is
the incomplete loss of RPE and the ability to rescue the
phenotype
through low-temperature rearing, implying that another protein,
or
proteins, can supplement for the loss of Yap. Based on our
observations, we believe Taz is responsible for this
compensation.
Although the levels of taz transcript in yap−/− embryos are not

increased, the yap−/− RPE phenotype is taz gene dosage-
dependent
and Taz protein was elevated in yap−/− mutants. That taz
mRNA
levels are unaffected is consistent with observations in yap−/−
cardiomyocytes, which also show taz gene dosage-dependent
phenotypes (Xin et al., 2013). The decreased taz mRNA levels
could be the result of increased nuclear localization of Taz,
which
negatively feeds back on taz transcription. Changes in the
stability
of Taz might contribute to the increased Taz protein levels
accompanying decreased taz mRNA. Taz contains a
phosphodegron site that is primed by Lats phosphorylation,
resulting in subsequent ubiquitylation and degradation (Liu et
al.,
2010). Potentially, the stability of Taz could be changed by a
decrease in phosphorylation of the phosphodegron in yap
mutants.
The following scenario may explain the partial loss of the RPE
phenotype of yap mutants. Within yap−/− embryos Taz stability
might be enhanced, but it might take time for translated Taz
protein
to reach sufficient levels for RPE specification. If so, those
progenitor cells that spread and migrate further to the periphery
prior
to differentiation may have time to stabilize Taz and
consequently
generate RPE (Fig. 8H). The progenitor cells in the central eye
differentiate at or near their site of origin and, consequently,
Taz
may have insufficient time to reach the levels necessary to
promote
RPE fate (Fig. 8H). Because RPE and NR differentiate from the

same pool of cells within the optic cup, the cells that fail to
adopt
RPE fate in yap−/− mutants might instead become NR
progenitors,
but it is also possible that these cells assume extra-ocular cell
type
fates (Fig. 8H).
Slowing development by low-temperature rearing might allow
sufficient Taz levels to accumulate, enabling compensatory Taz
activity within central progenitor cells, thus explaining the
rescue of
Table 1. yap−/−; taz−/− transplanted cells do not contribute to
the RPE
Genotype H2A-GFP+ NR Pigment+ RPE Retina/RPE ratio
RPE/retina (%) P-value
yap+/+; taz+/+ 70 30 2.3 42.9
yap+/+; taz+/– 37 8 4.6 21.6 0.154
yap+/+; taz–/– 31 10 3.1 32.3 0.544
yap+/–; taz+/+ 58 22 2.6 38.0 0.743
yap+/–; taz+/– 86 36 2.4 42.0 1
yap+/–; taz–/– 44 19 2.3 43.2 1
yap–/–; taz+/+ 33 3 11 9.1 0.011
yap–/–; taz+/– 47 9 5.2 19.1 0.057
yap–/–; taz–/– 23 0 – 0 0.001
P-values were obtained using Fischer’s exact comparison.
Table 2. Upregulated RPE/pigment-related transcripts in 36 hpf
NR overexpressing Yap S87A
Transcript ID Gene Fold change

ENSDART00000144551 bloc1s2 8.18
ENSDART00000047272 cx43 5.09
ENSDART00000061261 cx43 3.07
ENSDART00000143374 mitfa 2.99
ENSDART00000030887 slc45a2 2.86
ENSDART00000067479 stra6 2.83
ENSDART00000104832 hps5 2.75
ENSDART00000034248 rab32a 2.69
ENSDART00000132320 pmelb 2.68
ENSDART00000145138 dct 2.45
ENSDART00000074833 rx3 2.44
ENSDART00000004533 rpe65a 2.38
ENSDART00000108943 shroom2 2.34
ENSDART00000124194 slc24a5 2.23
ENSDART00000148817 oca2 2.20
ENSDART00000026017 bhlhe40 2.11
ENSDART00000017153 hps4 2.10
ENSDART00000110077 best1 1.91
ENSDART00000006538 otx1a 1.85
ENSDART00000123568 pmela 1.81
ENSDART00000122238 tyr 1.73
ENSDART00000133864 gpr143 1.69
ENSDART00000109822 hps1 1.68
ENSDART00000013835 bloc1s1 1.59
ENSDART00000059972 crabp2a 1.58
ENSDART00000039597 crabp2b 1.57
ENSDART00000049863 tfec 1.52
ENSDART00000139555 gpr143 1.52
ENSDART00000078594 tyrp1b 1.51
ENSDART00000050528 tyrp1a 1.43
ENSDART00000046268 pmelb 1.29
ENSDART00000126097 otx2 1.29
ENSDART00000025036 gap43 1.23
Values indicate fold change in Yap S87A-expressing eyes
compared

with sibling controls. All transcripts were significantly
upregulated based
on an adjusted P-value <0.05.
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yap−/− phenotypes by temperature shift. Consistent with this
idea,
low-temperature rearing does not rescue RPE when Taz is also
abrogated. Importantly, cell transplantations indicated that
yap−/−;
taz−/− double-homozygous mutant cells are completely

incapable of
generating RPE. Similarly, dominant-negative Yap or Taz
disrupts
RPE genesis whereas, conversely, overexpression of either Yap
or
Taz can drive RPE fate from optic cup progenitor cells.
We do not know the upstream mechanism controlling Yap/Taz
activity during RPE genesis, although it is likely that cross-talk
with
other signaling pathways is involved. WNT, BMP and TGFβ
signaling all control aspects of RPE/NR development through
the
control of Smads and β-catenin (Sinn and Wittbrodt, 2013;
Fuhrmann et al., 2014), both of which have been shown to
interact with Yap or Taz (Varelas et al., 2010; Azzolin et al.,
2012).
Consequently, it is possible Yap/Taz-Tead activity regulates the
transcription of RPE-specific genes in concert with the
transcription
factor targets of these pathways.
Yap and Taz have multiple binding partners within the
cytoplasm
and nucleus, the best-characterized of which are the Tead
transcription factors. Our data imply that Yap/Taz-Tead
interactions are responsible for RPE genesis. First, the
4xGTIIC:
FP Tead-responsive transgenic reporter is active early within
RPE
progenitor cells. Second, nuclear localized dominant-negative
Yap
and Taz proteins can cause RPE loss. Finally, loss of RPE was
observed with overexpression of the Yap-binding-deficient
Tead1a
Y417H form, and within eyes in the yap Tead-binding-mutant

allele. Despite the loss of RPE cells within yapΔTB/ΔTB
mutants, the
embryos maintained wild-type levels of yap mRNA and robust
levels of Yap protein. Loss of Tead binding by Yap in other
contexts
also results in similar phenotypes to yap loss-of-function and
Yap-
binding-deficient alleles of TEAD1 (Fossdal et al., 2004;
Williamson et al., 2014). SCRA patients carry an autosomal
dominant mutation in the Yap-binding domain of TEAD1 and
the
loss of central RPE in these patients mimics the phenotypes
observed in zebrafish yap−/− and Tead1a Y417H overexpression
embryos (Fossdal et al., 2004; Jonasson et al., 2007). Given
these
similarities, our data suggest that the congenital defects in the
RPE
of SCRA patients arise from autonomous Yap/Tead functional
deficits of cells within the optic cup, as in yap loss-of-function
zebrafish mutants.
As observed in cases of human mutations of YAP1 we see
phenotypic variability within and across our different zebrafish
yap
mutant genotypes. Notable differences occur when yap−/−
phenotypes are compared with yapnl13/nl13 phenotypes. The
loss
of RPE is less evident in yapnl13/nl13 mutants but there is a
striking
coloboma, similar to that observed in human patients with
YAP1
mutations (Williamson et al., 2014). We have not resolved why
coloboma is evident in this mutant, but one intriguing
possibility is
that it could be a consequence of compromised RPE generation

in
the ventral retina. This might introduce mechanical disruptions
to
the movements of cells lining the fissure that do not occur when
RPE generation is disrupted in the central retina. In this regard,
it is
interesting to note that recent studies of yap mutant medaka fish
suggest that this pathway is a crucial regulator of actomyosin
contractility (Porazinski et al., 2015). To address this issue will
require manipulation of Yap/Taz-Tead activity in different
populations of cells of the ventral retina to determine the cause
of
the colobomatous phenotype.
Our data reveal a role for Yap and Taz activity in regulating the
ability of optic vesicle cells to adopt RPE cell fate. The idea
that
combined Yap/Taz activity is crucial for RPE fate determination
is
consistent with recently published reports of Yki and Yap/Taz
influencing cell fate decisions in various other tissues (Nishioka
et al., 2009; Zhang et al., 2011; Judson et al., 2013; Yimlamai
et al.,
2014). Our data potentially have implications for the efficient
directed differentiation of stem cells to RPE fate – an important
goal,
given the initial success of stem cell-differentiated RPE cell
transplants into patients with macular degeneration (Schwartz
et al., 2015).
To conclude, in this study we revealed that Yap and Taz
influence
RPE cell fate in a gene dosage-dependent manner. The
underlying
mechanism is through a cell-autonomous nuclear interaction of

Yap/Taz with Tead transcription factors. The elucidation of this
basic mechanism offers a framework for continued investigation
into the upstream and downstream protein-protein and protein-
DNA
interactions in eye development. The mutant lines and
transgenic
resources generated in this study will also facilitate resolution
of the
mechanistic basis of ocular phenotypes seen in patients with
SCRA
and congenital retinal coloboma caused by aberrant Yap/Taz-
Tead
activity.
MATERIALS AND METHODS
Mutant generation
TALENs for Yap and Taz were generated by Cellectis
Bioresearch and
supplied as plasmids (pTAL.CMV-T7.013535, pTAL.CMV-
T7.013558,
pTAL.CMV-T7.013534 and pTAL.CMV-T7.01355). The left
yap TALEN
arm targeted exon 1 (5′-TGAGGTTGAGAAAGCTG-3′) and 15
nt
downstream in exon 1 was the right yap TALEN arm target
sequence (5′-
TTTGGCTCTGGCGGCGT-3′). The left taz TALEN arm
targeted exon 1
(5′-TGCCGCAGTCTTTCTTC-3′) and 15 nt downstream in exon
1 was the
right taz TALEN arm target sequence (5′-
TGCCGGGAGTGGGAGCC-3′).
mRNA was generated from the supplied plasmids using the
mMessage
mMachine and Poly(A) Tailing Kit (Ambion) and injected into
1- to 4-cell

stage embryos at 150 pg per TALEN arm. Offspring from the
yap or taz
TALEN-injected embryos were screened using restriction sites
within each
spacer region. Once founders were identified, offspring were
raised and
tested for allele heterozygosity. The nl13 allele was identified
in a forward
genetic screen of fish carrying N-ethyl-N-nitrosourea-induced
mutations
carried out by Alex Nechiporuk’s group (Oregon Health and
Science
University). Mapping and identification of the causative
mutation followed
standard procedures (Valdivia et al., 2011).
Genotyping
The Puregene Core Kit A (Qiagen) was used to extract genomic
DNA
(gDNA) from zebrafish tissue. Each gene region of interest was
PCR
amplified (supplementary material Table S4) and assessed for
the presence
or absence of a restriction site. The yap 4 bp deletion mutant
(mw48) was
detected by the addition of a TfiI restriction site, while the yap
21 bp (mw69)
and taz 5 bp (mw49) deletion mutants were detected by the loss
of HinfI.
Generation of plasmids and transgenic lines
All plasmids were generated using Gateway (Invitrogen) entry
clones and
recombination in conjunction with the Tol2 Kit (Kwan et al.,
2007). The
2.7 kb proximal upstream region of tfec was obtained through

PCR of
zebrafish gDNA and using recombination eGFP was inserted
downstream of
the −2.7 kb tfec promoter (−2.7 kb tfec:eGFP). RT-PCR was
performed to
generate Flag- or Myc-tagged full-length cDNA sequences for
zebrafish yap
and tead1a. A Tead-binding-deficient Yap (Yap S54A) was
generated using
QuikChange (Stratagene) site-directed mutagenesis. The Yap-
binding-
deficient Tead1a (Tead1a Y417H) mutant was made by
including
nucleotide changes within the 3′ PCR primer used for cloning.
Flag-Yap,
Flag-Yap S54A, Myc-Tead1a and Myc-Tead1a Y417H were
inserted
downstream of the CMV/SP6 promoter using recombination.
Yap, Yap
S87A, Taz, Myc-Tead1a Y417H and Yap and Taz dominant-
negative
constructs were generated as previously described (Miesfeld and
Link,
2014). Transposase mRNA was injected with the fully
assembled Tol2
constructs to generate each transgenic line (Kawakami, 2005).
Transgenic
and mutant lines are listed in supplementary material Table S5.
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Immunofluorescence
Standard methodology was used for whole-mount
immunofluorescence and
imaging (Clark et al., 2011). A 1:200 dilution of Yap rabbit
polyclonal (Cell
Signaling, 4912) or Taz/Yap (D24E4) rabbit monoclonal (Cell
Signaling,
8418) and a 1:800 dilution of Alexa 488 anti-rabbit (Invitrogen)
were used
for Yap and Taz detection. Nuclei were labeled with TO-PRO-3
(Molecular
Probes).
Transfection assay
HEK293 cells were grown in DMEM (Gibco) supplemented with
10% FBS
and penicillin and streptomycin at 37°C in 5% CO2. Cells were
transfected
with Lipofectamine 2000 (Invitrogen) according to the

manufacturer’s
instructions. Plasmids transfected included
pTol2:CMV/SP6:Flag-YapWT:
pA, pTol2:CMV/SP6:Flag-YapS54A:pA, pTol2:CMV/SP6:Myc-
Tead1aWT:
pA and pTol2:CMV/SP6:Myc-Tead1aY417H:pA.
Immunoprecipitations and western blotting
Cells were harvested 24 h after transfection in lysis buffer (50
mM Tris-HCl
pH 8.0, 150 mM NaCl, 1% NP-40) supplemented with a protease
inhibitor
cocktail (Roche), lysed in liquid nitrogen, centrifuged at 10,000
rpm (11,180 g)
for 10 min, and the supernatants used for immunoprecipitation
analysis.
Immunoprecipitations were performed with rabbit monoclonal
anti-Myc
(Cell Signaling, 2278) or rabbit monoclonal anti-Flag (Sigma-
Aldrich,
F7425) antibodies and protein G-sepharose beads (GE
Healthcare)
overnight on a rotator at 4°C. Proteins were eluted from the
beads by
boiling for 5 min in SDS sample buffer. Elutes were
fractionated by SDS-
PAGE (7.5% Mini-Protean TGX, Bio-Rad) and transferred to
Immobilon
FL PVDF membranes (Millipore). Membranes were incubated
for 1 h at
room temperature in Odyssey blocking buffer (LI-COR
Biosciences), and
incubated overnight at 4°C with anti-Myc or anti-Flag primary
antibodies
diluted 1:1000 in Odyssey blocking buffer, then with near
infrared

fluorescent secondary antibodies (IRDye 680RD and IRDye
800CW, LI-
COR Biosciences) for 1 h at room temperature. Proteins were
detected with
an Odyssey infrared imager (LI-COR Biosciences).
Equivalent numbers (n=20) of 2 dpf wild-type and yap−/− whole
embryos
were de-yolked (Link et al., 2006) and processed in lysis buffer
and
centrifuged as previously described. The supernatants were
boiled for 5 min
in SDS sample buffer and all subsequent steps were as described
above. Taz
was detected using a 1:1000 dilution of Taz/Yap (D24E4) rabbit
monoclonal (Cell Signaling, 8418).
In situ hybridization
Embryos were fixed in 4% paraformaldehyde at 48 hpf and
standard
methodology was followed as previously described (Thisse and
Thisse, 2004).
Proliferation analysis
Embryos were collected from incrosses of yap+/–; h2afz:GFP+
fish and raised
in 0.003% N-phenylthiourea (PTU) diluted in Instant Ocean fish
water at
28.5°C before analysis, and transferred to 100% fish water after
analysis.
Embryos were dechorionated at 14, 18 or 24 hpf, anesthetized in
3-amino
benzoic acid ethyl ester (Tricaine, Sigma-Aldrich), and mounted
in 1% low-
melt agarose (Fischer Scientific) in glass-bottom Petri dishes.
Both eye fields

were imaged for each embryo using confocal microscopy. After
imaging,
embryos were freed and raised individually in 48-well plates.
The genotype
of each embryo was determined by the loss of RPE phenotype at
48 hpf.
Mitotic cells for each image were counted using ImageJ
software (NIH). A
cell was counted as mitotic if a metaphase plate was clearly
visible in a single
or recently divided nucleus. This same analysis was performed
at 14 hpf for
−2.7 kb tfec:eGFP+ cells. To inhibit proliferation we treated
wild-type
embryos with 20 mM hydroxyurea (Sigma-Aldrich), 150 μM
aphidicolin
(Cayman Chemical) and 3% DMSO in fish water from 10.5-26
hpf.
Cell death analysis
Embryos were incubated at 28.5°C for 20 min in 20 ml 5 μg/ml
Acridine
Orange (Sigma-Aldrich) diluted in Instant Ocean embryo media
at 14, 18 or
24 hpf. After incubation, embryos were briefly washed four
times in PTU,
anesthetized in Tricaine and embedded in 1% low-melt agarose
in glass-
bottom Petri dishes and imaged by confocal microscopy.
Embryos were
freed and reared individually in a 48-well plate. Genotypes were
assessed
based on the loss of RPE phenotype. Cell death counts were
performed
using MetaMorph (Molecular Devices). Cell death was inhibited

by co-
injecting 150 μM tp53 morpholino (Robu et al., 2007) and 100
pg bcl-xl
mRNA (Sidi et al., 2008) into 1- to 2-cell stage embryos.
Quantitative real-time PCR analysis
All cDNA was generated using the Superscript III First-Strand
Synthesis
System for RT-PCR Kit (Invitrogen) per manufacturer’s
instructions and all
qRT-PCR was performed on a CFX96 and CFX Connect Real-
Time System
(Bio-Rad) using SsoAdvanced SYBR Green Supermix (Bio-
Rad). Analysis
of yap and taz transcripts was performed on whole embryos.
mRNA was
extracted from dechorionated wild-type (ZDR), yap−/− and
yapΔTB/ΔTB
embryos at 32 hpf using the RNeasy Plus Mini Kit (Qiagen) per
manufacturer’s instructions. qRT-PCR was performed using
equivalent
amounts of mRNA for four biological replicates of ten pooled
embryos for
ZDR and yap−/− embryos, while three biological replicates of
ten pooled
embryos were used for yapΔTB/ΔTB embryos. All biological
replicates were
run in triplicate for each transcript. The zebrafish housekeeping
gene ef1a
was used for normalization.
For verification of transcripts identified as upregulated in RNA-
seq,
whole eye tissue from 36 hpf embryos expressing Yap S87A or
non-

expressing siblings was pooled (15 embryos/30 eyes) and
mRNA was
extracted using TRIzol (Invitrogen). All transcripts were
measured in
triplicate for each of three independent biological replicates
analyzed. ef1a
was used for normalization.
Blastulae transplantation
Donor cells were transplanted and targeted into host embryos as
previously
described (Kemp et al., 2009). After transplantation, donor
embryos were
genotyped and host embryos raised individually in 48-well
plates containing
fish water. Retinal clones were scored between 1 and 2 dpf
based on the
presence of eGFP+ nuclei. RPE clones were assessed at 2 dpf
based on
pigmentation and the characteristic hexagonal shape of RPE
cells (Fig. 7).
Only eyes that contained H2A-GFP+ NR clones were assessed
for RPE
clones. Fischer’s exact test was used to compare the RPE/NR
ratio of each
mutant genotype combination with the wild-type ratio.
RNA-seq
Yap S87A and sibling control whole eyes were dissected at 36
hpf and
immediately frozen on dry ice until ∼60 pooled retinas were
obtained for
each genotype. RNA was purified as described (Uribe et al.,
2012), except
that RNA was eluted in a 50 μl final volume. RNA quality was
determined

using an Agilent BioAnalyzer. 50 bp single-read sequencing
was performed
in triplicate for each genotype using an Illumina HiSeq2000 at
VANTAGE
(Vanderbilt University). Sequencing results were analyzed by
VANGARD
(Vanderbilt University). RNA-seq reads were mapped to D.
rerio cDNA
sequences from Ensembl release 66. RNA-seq data have been
deposited at
GEO under accession GSE71681.
Acknowledgements
We thank Jon Bostrom, Peter Volberding and Rod Young for
assistance with
molecular biology; Emily Seminary for assistance with
supplementary material
Fig. S3; Pat Cliff, Bill Hudzinski and the UCL Fish Facility
team for zebrafish
husbandry; Dr James Lister for the dct in situ probe plasmid; Dr
Kristen Kwan and
Dr Masa Tada for the bcl-xl and yap-GFP mRNA plasmids,
respectively; and Alex
Nechiporuk for kindly providing the nl13 allele for analysis.
Competing interests
The authors declare no competing or financial interests.
Author contributions
J.B.M., G.G., J.C.B., S.W.W. and B.A.L. designed experiments.
J.B.M. and B.A.L.
co-wrote the paper with help from G.G. and S.W.W. J.B.M.,
B.S.C. and M.A.F.
conducted experiments for RNA-seq and validation. R.J.P.
performed sequence
analysis to identify the nl13 mutation. J.R.B. and J.B.M.

conducted the
immunoprecipitation assays and G.G. performed all experiments
using the nl13
allele. All authors helped edit the manuscript.
3031
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Funding
RNA-seq experiments were performed in VANTAGE
(Vanderbilt University,
Nashville, TN, USA), which is supported by the Vanderbilt
Ingram Cancer Center
[P30 CA68485], the Vanderbilt Vision Center [P30 EY08126]
and National Institutes

of Health/National Center for Research Resources (NIH/NCRR)
[G20 RR030956].
This project was supported by an NIH/National Eye Institute
(NEI) training grant
[T32EY014536 to J.B.M. and B.S.C.] to the Medical College of
Wisconsin (MCW)
vision research community; an NIH grant [R01EY014167] to
B.A.L.; a pilot project
grant from the MCW Cancer Center – Advancing a Healthier
Wisconsin (to B.A.L.);
an NEI core facilities grant [P30EY001931] to the vision
research community of
MCW; UK Medical Research Council and Wellcome Trust
grants to S.W.W. and
G.G.; and a Wellcome Trust career development fellowship to
R.J.P. Deposited in
PMC for immediate release.
Supplementary material
Supplementary material available online at
/DC1
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