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Biology
Unit 4
AQA
Ecology Definitions
Habitat – The place where an organism lives
Population – A group of organisms belonging to the same species
Community – All the populations of different organisms living and interacting
in the same space at the same time
Ecosystem – A community of living organisms and the abiotic factors which
affect them
Abiotic – The physical and chemical features of the environment
Biotic – The biological features of the environment (living)
Niche – A species role within it’s habitat
Adaptation – A feature that members of a species have to increase their
chance
of survival
Investigating
Populations
Quadrats:
- Set out 2 tape measure at right angles, forming the axes for the
chosen area
- Generate 2 random numbers (using calculator) to use as coordinates
- Place quadrat where co-ords meet
- Find mean number of species per quadrat
- Multiply by size of area being sampled
Transects:
- It’s a line through an area to be studied to identify changes through
an area

- Line Transects – a tape measure is placed along the transect and
the
species that touch the tape measure are recorded
- Belt Transects – quadrats are placed next to each other along the
transect to work out species frequency &
percentage
cover along a transect
Measuring Abundance
Quadrats:
- Have a known dimension
- Used to:
- Estimate population density
- Estimate % cover of an organism
- Estimate the frequency of an organism
Factors:
- Size of quadrat – More small quadrats = more representative results
- Number of quadrats – more quadrats = more reliable results
- Position of quadrat – must be placed randomly to avoid bias
At least 20 samples taken. Eventually a sample size is big enough that the
number of species doesn’t increase much more the sample is said to be
representative.
Mark-Release
Recapture
A known number of animals are caught and marked. They’re then released back.
Later another sample are caught and the number of marked individuals is recorded

Assumptions:
- No reproduction
- No migration
- Enough time for both marked & unmarked animals to mix
- Marking doesn’t affect behaviour
Variation in Population Size
Abiotic Factors:
- Affected by factors such as temperature, light, space, water etc…
- When conditions are ideal an organism will thrive and vice versa
Biotic Factors:
- Interspecific Competition:
-

Competition between different species

- Intraspecific Competition:
-

Competition between the same species

- Predation – Predator & Prey populations are linked
-

Prey increases, more food, so predator increases.
Predator eats prey, prey decreases as they’re eaten
Predator decreases due to lack of food
Predator peaks after prey
Human Populations
Population Growth = (BR + Immigration) – (DR + Emigration)
% Population Growth Rate =

Population Change
Population Start

x 100

Demographic Transition Model:
- Shows the change in BR, DR & population size over along period of time
Survival Curves
Show the percentage of all individuals that were born in a population that are still
alive at a given age.
Life Expectancy – is the age someone is expected to live to
- it’s the age at which 50% of the population are still alive

e.g. the life expectancy of this example is
81 as that is the age when 50% of the
population are still alive
Age-Sex Population
Pyramids
West Europe:
-

West Africa:
-

High BR
Short Life Expectancy
High DR
Developing Country

Lower BR
Long Life Expectancy
Lower DR
Developed Country
Ecosystem Definitions
Producer – They’re photosynthetic organisms that manufacture organic
substances using light energy, water and CO2
Consumer – They’re organisms that obtain their energy by feeding on other
organisms
Decomposers – When consumers & producers die, the energy can be used
by
organisms that break down the complex materials into single
components again
Food Chains – Describes a feeding relationship in which the producer are
eaten
by the primary consumers. They’re then eaten by secondary
consumer
Trophic Level – The level between each stage in the food chain
Food Web – More than one food chain linked together
Trophic Level

Grass  Sheep  Human
(Producer)

(1° Consumer)

(2° Consumer)
Energy Transfer Between
Trophic Levels
Little solar energy converted to chemical energy in PS:
- Some is reflected due to wrong wavelength/frequency/colour
- Doesn’t hit chlorophyll molecule
- Lost as heat during evaporation
Energy is lost along a food chain:
- Not all the organism is eaten
- Not all organism digested – lost in faeces
- Urine
- Heat in respiration
- Movement
- Birds & Mammals – energy used to maintain a constant body temperature
(homeostasis)
Not enough energy to support further trophic levels, so rarely more than 4
trophic levels present in a food chain
Net Primary Productivity
Gross Primary Productivity (GPP) – Amount of light energy that plants
convert
to chemical energy
Net Primary Productivity (NPP) – Total amount of energy stored in a plant
that
is available to the next trophic level
NPP = GPP - Respiration
Measured in
Energy
=
Transfer (%)

kJ m-2 Year -1
Energy after Transfer
X 100
Energy before Transfer
Production of ATP
•

ATP- Adenine TriPhosphate

•

Made from ADP + Pi

•

Energy stored in the phosphate bond

•

ATPase catalyses the breakdown of ATP into ADP + Pi

•

ATP synthase catalyses the production of ATP

•

The ADP + Pi is recycled and the process starts again
Properties:
• Small compound – easily transported around the
cell
• Easily broken down (Hydrolysed)
• Cell has instant energy supply
Photosynthesis
2 Photo Systems capture light in a chloroplast PSI (best at 700nm) & PSII (best at
680nm)
Thylakoid

Stroma Starch Grain

6CO2 + 6H2O + Energy = C6H12O6 + 6O2
Granum
Contains
Chlorophyll

Substomatal
Cavity

Inner &
Outer
membrane

Waxy Cuticle
Loop of DNA

Lamellae (Membrane
joining Thylakoids)

Absorption Spectrum

Number of
Chloroplasts

Upper Epidermis
Airy Cells,
lots of
space

Palisade Layer

Spongy Mesophyll
Lower Epidermis

Plants absorb red & blue
wavelengths only
reflecting green. It’s why
they’re green
LDS (Non-Cyclic Photophosphorylation)
Electron Acceptor

Electron Carrier

Photolysis Of Water:

2H2O = 4H+ + 4e- + O2
Requires a photon to split water
Occurs in the Thylakoids of
chloroplasts

Thylakoids adapted for their function:
•
•
•
•

Large SA, large area for attachment of chlorophyll, electron carriers and enzymes
Proteins in grana hold chlorophyll to allow max light intake
Granal membranes contain enzymes that help make ATP
Chloroplast contain DNA & Ribosomes to manufacture proteins for LDS quickly
Cyclic Photophosphorylation
Happens when lack of NADP
No light wasted
Only uses Photo System 1
Only ATP produced
LIS (Calvin Cycle)
In Stroma
RuBp – Ribulose
Bisphosphate
TP – Triose Phosphate
(GALP)
GP – Glycerate 3-Phosphate
RUBISCO – Enzyme used in
CO2 Fixation

ATP and rNADP from LDS
6 Cycles = 1 Glucose
Molecule
Respiration
C6H12O6 + 6O2 = 6CO2 + 6H2O + Energy

1. Glycolysis:
• Makes Pyruvate from Glucose
• In cytoplasm
• Anaerobic Process
• Net Yield of 2ATP
Dehydrogenation – Removal of H2
- Using dehydrogenase enzyme

Substrate Level Phosphorylation
- ADP + Pi  ATP
2. Link Reaction:
•
•
•

Pyruvate oxidised by removing H
Acetyl CoEnzyme A produced
Per Pyruvate a CO2 molecule produced

Decarboxylation – Removal of CO2
- Using Decarboxylase enzyme

Pyruvate + NAD + CoA = Acetyl CoA + rNAD + CO2
3. Krebs Cycle:
•
•
•

Acetyl CoA + oxaloacetate (4C) = Citrate
Citrate converted to 5C compound ( 2H+ & CO2 removed)
5C to 4C Produces:
• 2 x rNAD
• ATP
NAD – Nicotinamide Adenine Dinucleotide
• rFAD
FAD – Flavine Adenine Dinucleotide
• CO2
Electron Transfer Chain
When rFAD & rNAD are oxidised they release 2H & 2eElectrons used in transfer chain Hydrogen used in chemiosmosis
Energy/ATP produced in ETC is used to power chemiosmosis

Oxygen is the last electron acceptor.
O2 + 2e- + 2H  H2O
Chemiosmosis
In Photosynthesis & Respiration Energy (ATP) from ETC used to power Chemiosmosis
If ATP synthase
not present energy
lost in the form of
Heat instead of
forming ATP

Electro – Chemical
Gradient

Active Transport
Respiration
Anaerobic Respiration
Instead of pyruvate being converted into Acetyl CoA it’s
converted into ethanol (in plants and yeast) and lactic acid (in
animals and some bacteria)

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Unit 4 A2 Biology Notes AQA

  • 2. Ecology Definitions Habitat – The place where an organism lives Population – A group of organisms belonging to the same species Community – All the populations of different organisms living and interacting in the same space at the same time Ecosystem – A community of living organisms and the abiotic factors which affect them Abiotic – The physical and chemical features of the environment Biotic – The biological features of the environment (living) Niche – A species role within it’s habitat Adaptation – A feature that members of a species have to increase their chance of survival
  • 3. Investigating Populations Quadrats: - Set out 2 tape measure at right angles, forming the axes for the chosen area - Generate 2 random numbers (using calculator) to use as coordinates - Place quadrat where co-ords meet - Find mean number of species per quadrat - Multiply by size of area being sampled Transects: - It’s a line through an area to be studied to identify changes through an area - Line Transects – a tape measure is placed along the transect and the species that touch the tape measure are recorded - Belt Transects – quadrats are placed next to each other along the transect to work out species frequency & percentage cover along a transect
  • 4. Measuring Abundance Quadrats: - Have a known dimension - Used to: - Estimate population density - Estimate % cover of an organism - Estimate the frequency of an organism Factors: - Size of quadrat – More small quadrats = more representative results - Number of quadrats – more quadrats = more reliable results - Position of quadrat – must be placed randomly to avoid bias At least 20 samples taken. Eventually a sample size is big enough that the number of species doesn’t increase much more the sample is said to be representative.
  • 5. Mark-Release Recapture A known number of animals are caught and marked. They’re then released back. Later another sample are caught and the number of marked individuals is recorded Assumptions: - No reproduction - No migration - Enough time for both marked & unmarked animals to mix - Marking doesn’t affect behaviour
  • 6. Variation in Population Size Abiotic Factors: - Affected by factors such as temperature, light, space, water etc… - When conditions are ideal an organism will thrive and vice versa Biotic Factors: - Interspecific Competition: - Competition between different species - Intraspecific Competition: - Competition between the same species - Predation – Predator & Prey populations are linked - Prey increases, more food, so predator increases. Predator eats prey, prey decreases as they’re eaten Predator decreases due to lack of food Predator peaks after prey
  • 7. Human Populations Population Growth = (BR + Immigration) – (DR + Emigration) % Population Growth Rate = Population Change Population Start x 100 Demographic Transition Model: - Shows the change in BR, DR & population size over along period of time
  • 8. Survival Curves Show the percentage of all individuals that were born in a population that are still alive at a given age. Life Expectancy – is the age someone is expected to live to - it’s the age at which 50% of the population are still alive e.g. the life expectancy of this example is 81 as that is the age when 50% of the population are still alive
  • 9. Age-Sex Population Pyramids West Europe: - West Africa: - High BR Short Life Expectancy High DR Developing Country Lower BR Long Life Expectancy Lower DR Developed Country
  • 10. Ecosystem Definitions Producer – They’re photosynthetic organisms that manufacture organic substances using light energy, water and CO2 Consumer – They’re organisms that obtain their energy by feeding on other organisms Decomposers – When consumers & producers die, the energy can be used by organisms that break down the complex materials into single components again Food Chains – Describes a feeding relationship in which the producer are eaten by the primary consumers. They’re then eaten by secondary consumer Trophic Level – The level between each stage in the food chain Food Web – More than one food chain linked together Trophic Level Grass  Sheep  Human (Producer) (1° Consumer) (2° Consumer)
  • 11. Energy Transfer Between Trophic Levels Little solar energy converted to chemical energy in PS: - Some is reflected due to wrong wavelength/frequency/colour - Doesn’t hit chlorophyll molecule - Lost as heat during evaporation Energy is lost along a food chain: - Not all the organism is eaten - Not all organism digested – lost in faeces - Urine - Heat in respiration - Movement - Birds & Mammals – energy used to maintain a constant body temperature (homeostasis) Not enough energy to support further trophic levels, so rarely more than 4 trophic levels present in a food chain
  • 12. Net Primary Productivity Gross Primary Productivity (GPP) – Amount of light energy that plants convert to chemical energy Net Primary Productivity (NPP) – Total amount of energy stored in a plant that is available to the next trophic level NPP = GPP - Respiration Measured in Energy = Transfer (%) kJ m-2 Year -1 Energy after Transfer X 100 Energy before Transfer
  • 13. Production of ATP • ATP- Adenine TriPhosphate • Made from ADP + Pi • Energy stored in the phosphate bond • ATPase catalyses the breakdown of ATP into ADP + Pi • ATP synthase catalyses the production of ATP • The ADP + Pi is recycled and the process starts again Properties: • Small compound – easily transported around the cell • Easily broken down (Hydrolysed) • Cell has instant energy supply
  • 14. Photosynthesis 2 Photo Systems capture light in a chloroplast PSI (best at 700nm) & PSII (best at 680nm) Thylakoid Stroma Starch Grain 6CO2 + 6H2O + Energy = C6H12O6 + 6O2 Granum Contains Chlorophyll Substomatal Cavity Inner & Outer membrane Waxy Cuticle Loop of DNA Lamellae (Membrane joining Thylakoids) Absorption Spectrum Number of Chloroplasts Upper Epidermis Airy Cells, lots of space Palisade Layer Spongy Mesophyll Lower Epidermis Plants absorb red & blue wavelengths only reflecting green. It’s why they’re green
  • 15. LDS (Non-Cyclic Photophosphorylation) Electron Acceptor Electron Carrier Photolysis Of Water: 2H2O = 4H+ + 4e- + O2 Requires a photon to split water Occurs in the Thylakoids of chloroplasts Thylakoids adapted for their function: • • • • Large SA, large area for attachment of chlorophyll, electron carriers and enzymes Proteins in grana hold chlorophyll to allow max light intake Granal membranes contain enzymes that help make ATP Chloroplast contain DNA & Ribosomes to manufacture proteins for LDS quickly
  • 16. Cyclic Photophosphorylation Happens when lack of NADP No light wasted Only uses Photo System 1 Only ATP produced
  • 17. LIS (Calvin Cycle) In Stroma RuBp – Ribulose Bisphosphate TP – Triose Phosphate (GALP) GP – Glycerate 3-Phosphate RUBISCO – Enzyme used in CO2 Fixation ATP and rNADP from LDS 6 Cycles = 1 Glucose Molecule
  • 18. Respiration C6H12O6 + 6O2 = 6CO2 + 6H2O + Energy 1. Glycolysis: • Makes Pyruvate from Glucose • In cytoplasm • Anaerobic Process • Net Yield of 2ATP Dehydrogenation – Removal of H2 - Using dehydrogenase enzyme Substrate Level Phosphorylation - ADP + Pi  ATP
  • 19. 2. Link Reaction: • • • Pyruvate oxidised by removing H Acetyl CoEnzyme A produced Per Pyruvate a CO2 molecule produced Decarboxylation – Removal of CO2 - Using Decarboxylase enzyme Pyruvate + NAD + CoA = Acetyl CoA + rNAD + CO2
  • 20. 3. Krebs Cycle: • • • Acetyl CoA + oxaloacetate (4C) = Citrate Citrate converted to 5C compound ( 2H+ & CO2 removed) 5C to 4C Produces: • 2 x rNAD • ATP NAD – Nicotinamide Adenine Dinucleotide • rFAD FAD – Flavine Adenine Dinucleotide • CO2
  • 21. Electron Transfer Chain When rFAD & rNAD are oxidised they release 2H & 2eElectrons used in transfer chain Hydrogen used in chemiosmosis Energy/ATP produced in ETC is used to power chemiosmosis Oxygen is the last electron acceptor. O2 + 2e- + 2H  H2O
  • 22. Chemiosmosis In Photosynthesis & Respiration Energy (ATP) from ETC used to power Chemiosmosis If ATP synthase not present energy lost in the form of Heat instead of forming ATP Electro – Chemical Gradient Active Transport
  • 24. Anaerobic Respiration Instead of pyruvate being converted into Acetyl CoA it’s converted into ethanol (in plants and yeast) and lactic acid (in animals and some bacteria)