Acclimatization in micropropgation
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The document outlines the stages of micropropagation, including the selection of mother plants, culture establishment, shoot multiplication, rooting, and hardening. Hardening involves gradually adapting plantlets to environmental conditions to enhance growth and reduce mortality, while acclimatization prepares the plants for field conditions. Success in commercial micropropagation relies on large-scale, cost-effective transfers with high survival rates.
Acclimatization in micropropgation
- 1. AMITY INSTITUTE OF BIOTECHNOLOGY Transfer and establishment of whole plants in soil (Hardening) AKANKSHA SINGH
- 2. Micropropagation and its stages: Stage 0 selection of mother plant and its maintenance stage I initiation and establishment of culture Stage II multiplication of shoot or rapid somatic embryo formation Stage III invitro rooting of shoot or germination of somatic embryo Stage IV transfer of platelets to sterilized soil for hardening • Micropropagation is the artificial process of producing plants vegetatively through tissue culture or cell culture techniques. • Transfer and establishment of whole plants in soil (HARDENING) is one the stages of micropropagation.
- 3. Stages involved in hardening
- 4. HARDENING • Hardening is gradual exposure of plantlets to an environmental conditions. • Challenges faced by micropropagation: a) Unable to compete with soil microbes and to cope with the environmental conditions b) Altered morphology, anatomy and physiology. • Agenda behind hardening in micro-propagation: a) increase growth and reduce mortality in plantlets b) efforts are focused on the control of both physical and chemical environment c) bio-hardening of micro-propagated plantlets.
- 5. ACCLIMATIZATION • Acclimatization: process of gradual hardening of the cultured plants from laboratory to field so that the regenerated plants should adjust to field conditions. • In this stage, the plantlets are established in the soil. • The shoots from the laboratory are transferred to a greenhouse under controlled conditions of temperature, humidity and light. • The ultimate success of micropropagation on a commercial scale depends on the ability to transfer plants out of culture on: a) a large scale b) at low cost c) with high survival rates.
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