Learn IL-6 in 3 minutes | Interleukin 6
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The document provides quick facts about interleukin 6 (IL-6), including its cytokine properties, structure, and functions, as well as its role in inflammatory and autoimmune diseases. It discusses Western blot analysis and expected band sizes, highlighting IL-6's identification as a B cell stimulatory factor and its medical significance. Additional resources and images related to IL-6 and Western blot results are also referenced.
Learn IL-6 in 3 minutes | Interleukin 6
- 1. www.bosterbio.com Quick facts, control designs, Western Blot MW. Info you can use to find the best IL6 antibody. IL-6 IN 3 MINUTES
- 2. FACTS 01. Function, structure, etc. TISSUES 02. Expressing tissues/cells WESTERN BLOT 03. Expected band sizes HISTORY 04. …and medical significance TABLE OF CONTENTS
- 3. AlphaFold predicted structure Image source: https://alphafold.ebi.ac.uk/entry/P05231 ● Interleukin 6 (IL-6) ● Cytokine with a wide variety of biological functions ● Has pro- and anti- inflammatory properties ● Size: 212 amino acids ● Western blot: ~24 kDa 01. FACTS
- 4. 02. TISSUES Image source: https://www.proteinatlas.org/ENSG00000136244-IL6/tissue
- 5. 8087 images available on PMC: https://www.ncbi.nlm.nih.gov/pmc/?term=il-6+western+blot&report=imagesdocsum After reviewing several WB images, we observe one IL-6 band at ~ 24 kDa. 03. IL-6 WESTERN BLOT PMC3580035 Figure 2 PMC4107025 Figure 3 R&D Systems AF506 Boster Bio IL-6 Antibody (PA1700, Rabbit Polyclonal) PMC5817102 Figure 4 PMC5522095 Figure 6
- 6. IL-6 was originally identified as a B cell stimulatory factor initiating IgG production. 04. HISTORY Anti-IL6 therapy is a new strategy in the inflammatory autoimmune diseases and cancer. 1986 CLINICAL POTENTIAL
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Editor's Notes
- #2: Does your next experiment involve Interleukin 6? This video is for scientists who want to run Western blot, IHC, flow or other immunoassays against IL6.
- #3: We will cover some basic facts, protein expression, and interleukin 6’s expected behavior in Western blot. All information in this video is based on public information and no proprietary experimental evidence was used.
- #4: Topic 1: Quick facts about IL6. Interleukin-6 is a cytokine with a wide variety of biological functions in immunity, hematopoiesis, tissue regeneration, and metabolism. IL6 is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through IL6 receptor alpha. Rapid production of interleukin 6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. Interleukin 6 is 212 amino acids long and runs about 24 kilodaltons on Western blot.
- #5: Topic 2: Where is interleukin 6 expressed? High RNA expression levels of IL6 is found in the brain, lungs, adipose tissue, bone marrow, and skeletal muscle cells, with the highest levels detected in the urinary bladder. They are also expressed at lower levels in the kidney, liver, thyroid gland, pancreas, skin, prostate and retina. Elevated levels of the encoded protein have been found in virus infections, including COVID-19. You can find more information on protein atlas dot org to check out which cell lines express interleukin 6 and which ones don’t. This information will come in handy when designing positive and negative controls for western blot and immunohistochemistry.
- #6: Topic 3: Interleukin 6 in Western blotting. Antibody specificity can make or break an experiment. After looking through Western blot images from publications and antibody companies, we can see that typically one band at around 24 kilodaltons can be expected in an IL6 Western blot. Check out the PMC link for more images, which is also provided in the description box.
- #7: Topic 4: Some interesting facts about IL6. Interleukin 6 was first discovered in 1986 as a T cell-derived lymphokine that induces the final maturation of B cells into antibody-producing cells. IL6 can simultaneously elicit distinct or even contradictory physiopathological processes. When taking into account all the accumulated basic research on the various aspects of this molecule, therapeutic strategies to inhibit IL6 signaling have proven effective in treating experimental models of autoimmune and chronic inflammatory diseases.